All chemicals were from Sigma, Inc. Bacterial growth media and media supplements were from HiMedia Laboratories, Inc., Mumbai, India. Phusion polymerase for colony PCR was purchased from New England Biolabs Inc., United States. GroE expression plasmids, pBAD-GSL and pTrc-GSL were generated by cloning GroE operon into NcoI and HindIII sites on plasmids pBAD24 (Guzman et al., 1995 (link)) and pTrc99A (Amann et al., 1988 (link)), respectively. The groE conditional mutant strain, E. coli LG6, was a kind gift from Arthur Horwich, Yale University, United States (Horwich et al., 1993 (link)). This strain produces GroE at levels similar to the wildtype at 30°C upon induction (Supplementary Figure 1 ). Oligonucleotide primers were purchased from Integrated DNA Technologies, Inc., Coralville, IA, United States.
Phusion polymerase for colony pcr
Manufactured by New England Biolabs
Sourced in India, United States
Phusion polymerase is a high-fidelity DNA polymerase used for colony PCR. It has proofreading activity and can generate long amplicons with high accuracy.
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Lab products found in correlation
2 protocols using phusion polymerase for colony pcr
1
GroE Expression Plasmid Generation
2
Cloning and Characterization of GroE Operons
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Materials, Plasmids, Bacterial Strains and Growth Conditions. All chemicals were from Sigma, Inc. Bacterial growth media and media supplements were from HiMedia Laboratories, Inc., Mumbai, India. Phusion polymerase for colony PCR was purchased from New England Biolabs Inc, USA. GroE expression plasmids, pBAD-GSL and pTrc-GSL were generated by cloning GroE operon into NcoI and HindIII sites on plasmids pBAD24 (Guzman et al., 1995) and pTrc99A (Amann et al., 1988) , respectively. The groE conditional mutant strain, E. coli
LG6, was a kind gift from Arthur Horwich, Yale University, USA (Horwich et al., 1993) . This strain produces GroE at levels similar to the wildtype at 30 °C (Chapman et al., 2006) .
Oligonucleotide primers were purchased from Integrated DNA Technologies, Inc., Coralville, IA, USA.
LG6, was a kind gift from Arthur Horwich, Yale University, USA (Horwich et al., 1993) . This strain produces GroE at levels similar to the wildtype at 30 °C (Chapman et al., 2006) .
Oligonucleotide primers were purchased from Integrated DNA Technologies, Inc., Coralville, IA, USA.
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