Prelamin a

The antibodies used for immunofluorescence experiments were rat anti-LAMP1 (#1D4B, DSHB), rabbit anti-Aβ42 (#700254, Invitrogen), rabbit anti-BACE1 (#ab108394, Abcam), rabbit anti-βIII tubulin (#ab18207, Abcam). For immunoblotting, the antibodies used were rabbit anti-actin (#926–42,210, LI-COR), rabbit anti-p44/42 MAPK (pErk1/2) (#CS9101, Cell Signaling Technology), rabbit anti-44/42 (Erk1/2) (#CS137F5, Cell Signaling Technology), rabbit anti-LAMP1 (#CS3234, Cell Signaling Technology), mouse anti-BACE (3D5; Zhao et al., 2007), mouse anti-APP (6E10) (#803001, BioLegend), rabbit anti-p-tau (ser404) (#CS20194, Cell Signaling Technology), mouse anti-Tau1 (#MAB3420, Millipore Sigma), prelamin-A, (#MABPT858, Millipore Sigma) and mouse anti-HDJ-2 (#sc-59,554, Santa Cruz). Anti-rabbit or -mouse secondary antibodies from Vector Laboratories were used for immunoblots.

Cells were washed with cold PBS, lysed, and subjected to SDS‐PAGE using 4%–12% Bis‐Tris gels. Separated proteins were transferred to polyvinylidene fluoride membranes, which were blocked and incubated overnight at 4°C with anti‐rabbit primary antibodies (p21^WAF1, Cell Signaling #2947, 1:1000; p‐p53, Cell Signaling #9289, 1:1000; HMGB1, abcam# ab18256, 1:2000; prelamin A, Millipore Sigma #mabt858, 1:500; β‐actin, Sigma‐Aldrich #A2228, 1:10000). Membranes were washed and incubated with horseradish peroxidase‐conjugated (1:5000, Cell Signaling) secondary antibodies for 45 min at room temperature and washed again. Signals were detected by enhanced chemiluminescence.