Anti sirt2

Tenovin-6 (chemical structure, Fig. 1A) was purchased from Cayman Chemical (Ann Arbor, MI). Annexin V–FITC and N-acetylcysteine (NAC) were from Sigma-Aldrich (Shanghai, China). CM-H2DCF-DA and antibody against cytochrome c oxidase subunit II (COXII) were purchased from Invitrogen (Shanghai, China). Antibodies against SIRT1 (H-300), p53 (DO-7), Bax, DVL1, DVL3, β-catenin, cyclin D1 (C-20), survivin and c-Myc were from Santa Cruz Biotech. (Santa Cruz, CA). Antibody against active-β-catenin was from Millipore and the fluorescent secondary antibody was purchased from life technologies (Eugene. OR). Antibodies against PARP (clone 4C10-5), caspase-3, cytochrome c (clone 6H2.B4), XIAP, Bcl-2, phospho-GSK3β (Y216) were purchased from BD Biosciences (San Jose, CA). Antibodies against K382-acetyl-p53, DVL2, phospho-GSK3β (S9), MMP2 and MMP9 were from Cell Signaling Tech. (Beverly, MA). Anti-SIRT2 was purchased from Atlas Antibodies. Anti-mouse immunoglobulin G and anti-rabbit immunoglobulin G fluorescent-conjugated secondary antibodies were from LI-COR Biotechnology (Nebraska, USA).

Tenovin-6 was purchased from Cayman Chemical (Ann Arbor, MI). Antibodies against SIRT1 (H-300), p53 (DO-7), cyclin D1 (C-20), Mcl-1 (S-19), and proliferating cell nuclear antigen (PCNA) were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against PARP (clone 4C10-5), caspase-3, XIAP, and anti-CD19 conjugated with phycoerythrin were from BD Biosciences (San Jose, CA). Antibodies against K382-acetyl-p53 and c-Myc were from Cell Signaling Technology (Beverly, MA). Anti-SIRT2 was purchased from Atlas Antibodies. The CD133 MicroBead Kit including anti-CD133 conjugated with APC was from Miltenyi Biotec, Inc. (Shanghai, China). Anti-mouse immunoglobulin G and anti-rabbit immunoglobulin G horseradish peroxidase-conjugated secondary antibodies were from Pierce Biotechnology (Rockford, IL).