Soluble and fibrillar peptides prepared as described above were solubilized in 0.01 M sodium phosphate buffer (pH 7.4) containing 0.01% (w/v) NaN3 to a concentration of 0.1–4 μM. Immediately, 10 μl of a sample was added to a freshly cleaved layer of mica (Ted Pella, Inc., Redding, CA.) and allowed to air dry. AFM measurements were carried out on dry peptide samples with silicon nitride NP-S20 tips (Veeco Metrology, Inc., Santa Barbara, CA) using a MultiMode Scanning Probe Microscope (MM SPM) with a NanoScope IV controller. A high resolution J-type scanner was used to scan the peptide surface. Images were collected by raster scanning across a 10 μm2 area at 1024 samples (pixels) per line at a rate of 3.70 Hz. Images were processed with NanoScope Software (Veeco Metrology, Inc.).
Multimode scanning probe microscope mm spm
Manufactured by Veeco
The MultiMode Scanning Probe Microscope (MM SPM) is a high-performance lab equipment used for surface characterization and analysis. It employs scanning probe microscopy techniques to obtain detailed information about the topography and properties of samples at the nanoscale. The MM SPM is designed to provide precise measurements and high-resolution imaging capabilities for a wide range of applications.
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Lab products found in correlation
2 protocols using multimode scanning probe microscope mm spm
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Atomic Force Microscopy of Peptides
2
Atomic Force Microscopy of Peptides
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Soluble and fibrillar peptides prepared as described above were solubilized in 0.01 M sodium phosphate buffer (pH 7.4) containing 0.01% (w/v) NaN3 to a concentration of 0.1–4 μM. Immediately, 10 μl of a sample was added to a freshly cleaved layer of mica (Ted Pella, Inc., Redding, CA.) and allowed to air dry. AFM measurements were carried out on dry peptide samples with silicon nitride NP-S20 tips (Veeco Metrology, Inc., Santa Barbara, CA) using a MultiMode Scanning Probe Microscope (MM SPM) with a NanoScope IV controller. A high resolution J-type scanner was used to scan the peptide surface. Images were collected by raster scanning across a 10 μm2 area at 1024 samples (pixels) per line at a rate of 3.70 Hz. Images were processed with NanoScope Software (Veeco Metrology, Inc.).
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