Aurkb

Manufactured by Santa Cruz Biotechnology

Sourced in United States

AURKB is a lab equipment product offered by Santa Cruz Biotechnology. It is used to detect the presence and expression levels of the Aurora Kinase B protein, which is involved in cell division and chromosome segregation.

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Lab products found in correlation

2 protocols using aurkb

Elucidating BMP-2 Signaling Pathways

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Recombinant human BMP-2 was obtained from Dae Woong Pharmaceuticals (Seoul, South Korea). The BMP-2 antibody was aquired from Abcam (Cambridge, UK) and p-Smad1/5/8, β-catenin, p-β-catenin, ERK1/2, and p-ERK1/2 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies for BMPRII, c-Myc, AURKA, AURKB, and GAPDH were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). β-catenin small interfering RNA (siRNA) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA) and the aurora kinase A and B inhibitor (AURK inhibitor) was obtained from TOCRIS Bioscience (Bristol, UK).

Lee K.B., Jin H., Ye S., Park B.H, & Kim S.M. (2016). Recombinant human bone morphogenetic protein-2 inhibits gastric cancer cell proliferation by inactivating Wnt signaling pathway via c-Myc with aurora kinases. Oncotarget, 7(45), 73473-73485.

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Immunohistochemical Analysis of Aurora Kinases

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Three millimeters tumor sections were incubated with commercial rabbit polyclonal antibodies against AURKA, AURKB, and AURKC (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1/100 dilution overnight at 4°C. Then, the sections were conjugated with horseradish peroxidase antibody (1:500 dilution; Santa Cruz Biotechnology) at room temperature for 2 h, then covered by 3, 3-diaminobenzidine (Vector Laboratories, Burlingame, CA, USA), and slides were mounted with Vectashield mounting medium (Vector Laboratories). Subsequently, all fields were observed under light microscopy (Olympus 600 auto-biochemical analyzer, Tokyo, Japan). Control experiments without primary antibody demonstrated that the signals observed were specific.

Hu T., Wang X., Xia Y., Wu L., Ma Y., Zhou R, & Zhao Y. (2022). Comprehensive analysis identifies as a critical prognostic prediction gene in breast cancer. Chinese Medical Journal, 135(18), 2218-2231.

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