Tyramine (97%), histamine (98%), putrescine (99%), and cadaverine (99%) as main standards were individually ordered from Fluka (Buchs, Switzerland). Hydrochloric acid (37%), 1-octanol, acetonitrile, sodium chloride, acetone, potassium hydroxide (KOH), ethanol, methanol, 2-nitrophenyl octyle ether (NPOE), dipotassium hydrogen phosphate (K2HPO4), potassium hexaferrocyanide and zinc acetate were purchased from Merck (Darmstadt, Germany). Isobutyl chloroformate was obtained from Sigma-Aldrich (St. Louis, MO, USA). Ten non-alcoholic beer samples were randomly purchased from different supermarkets (Tehran, Iran).
Isobutyl chloroformate
Manufactured by Merck Group
Sourced in United States
Isobutyl chloroformate is a chemical compound used as a laboratory reagent. It is a colorless, volatile liquid with a pungent odor. Isobutyl chloroformate is commonly utilized in organic synthesis reactions, particularly in the formation of carbamates and other derivatives.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Methanol, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (3 mentions)
Trichloroacetic acid, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
N methylmorpholine, by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Zinc acetate, by Merck Group (1 mentions)
Potassium hexaferrocyanide, by Merck Group (1 mentions)
Potassium hydroxide koh, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
1 octanol, by Merck Group (1 mentions)
Thioctic acid, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
4 methylmorpholine, by Merck Group (1 mentions)
Human k2edta plasma, by BioIVT (1 mentions)
13 protocols using isobutyl chloroformate
1
Biogenic Amines Analysis in Non-Alcoholic Beer
2
Preparation of Amino Acid Derivatives
3
Synthesis and Characterization of Gold Nanoparticles
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Isobutyl chloroformate, thioctic acid, 4-methylmorpholine (MMP), tetrahydrofuran (THF), 2-cyano-6-aminobenzothiazole, tetrachloroauric acid trihydrate (HAuCl4·3H2O), cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) were purchased from Sigma-Aldrich (Shanghai, China). Silver nitrate (AgNO3), hydrochloric acid (HCl), ascorbic acid (AA), and trisodium citrate were purchased from Aladdin Chemistry Co. Ltd (Shanghai, China). Polyethyleneglycol (PEG, MW 5000) was obtained from Jenkem Technology Co. Ltd (Beijing, China). All chemicals were used without further purification, unless otherwise stated.
4
Quantitative Analysis of N-Acetylputrescine
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N-acetylputrescine reference standard, Bovine Serum Albumin (BSA), Trichloroacetic acid (TCA), Formic Acid (FA) and Isobutyl Chloroformate (IBCF) were obtained from Sigma (St. Louis, MO, USA). Optima LC/MS Grade of Acetonitrile, Water, and Sodium carbonate were purchased from Fisher Scientific (Pittsburgh, PA USA). Human K2-EDTA Plasma was supplied by BIOIVT (Westbury, NY, USA).
5
Biogenic Amine Profiling in Human Serum
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Biogenic amines, i.e. 1,3-diaminopropane (1,3-dap, ≥99%), putrescine (put, ≥99%), cadaverine (cad, ≥99%), N-acetyl-putrescine (N-actput, ≥98%), spermidine (spd, ≥98%), spermine (sp, ≥99%), N1-acetyl-spermine (N1-actsp, ≥97%), and l -ornithine (orn, ≥99%), along with four isotopically labeled internal standards d8-putrescine (d8-put, ≥99%), 13C5-ornithine (13C5-orn, 95%), d8-spermin (d8-sp, 95%), and 13C4-spermidine (13C4-spd, ≥95%), as well as acetonitrile and methanol (both HPLC gradient grade), trichloroacetic acid (TCA, ≥99%), sodium carbonate (≥99%), hydrochloric acid (fuming, 37%), acetic acid (99.8–100.5%), isobutyl chloroformate (≥99%), and human serum from clotted human male whole blood, sterile-filtered (mycoplasma tested, virus tested), were purchased from Sigma-Aldrich (Vienna, Austria). Phosphate buffered saline (PBS buffer, pH 7.2–7.3) was purchased from Apotheke LKH–Univ. Klinikum Graz, Austria. Ultra-pure water purified by a Milli-Q Gradient system (resistivity >18 MΩ cm; Millipore, Bedford, USA) was used for all experiments.
6
Peptide and Oligonucleotide Synthesis Protocols
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Fmoc–Leu–Ala–Gly–Gly and Fmoc–Gly–Pro–Leu–Gly–Leu–Ala–Gly–Gly peptides were purchased from Lipopharm.pl (Zblewo, Poland). Oligonucleotides 5′-CGT ACG CGT ACG CGT ACG CG-3′ and 5′-CGC GTA CGC GTA CGC GTA CG-3′ were purchased from Future Synthesis (Poznań, Poland). Collagenase IV and Hank’s Balanced Salt Solution (HBSS), with calcium and magnesium, were purchased from ThermoFisher Scientific (Walthman, MA, USA). Acetonitrile (ACN), 4-aminophenylmercuric acetate (APMA), dichloromethane (DCM), 2,5-dihydroxybenzoic acid (DHB), N,N-dimethylformamide (DMF), doxorubicin hydrochloride, formic acid, isobutyl chloroformate, methanol, sodium hydroxide, tetrahydrofuran (THF), triethylamine (TEA), and Tris-HCl were purchased from Sigma-Aldrich (St. Louis, MO, USA). Column chromatography was performed using silica gel NORMASIL 60 (40–63 mesh, VWR Chemicals, Radnor, PA, USA). Thin-layer chromatography was performed using silica plates, 60G, F254 (Sigma-Aldrich, St. Louis, MO, USA).
7
Antimalarial Compound Synthesis and Screening
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N-methyl morpholine (NMM), 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), trifluoroacetic acid (TFA), Ccm (diferuloyl methane), isobutyl chloroformate (IBCF), methanol and DL-threo-β-Phenylserine was purchased from Sigma–Aldrich (St. Louis, MO, USA). N-[(tert-butoxy)carbonyl]-l -methionine, l -phenylalanine, Anhydrous sodium sulfate and citric acid were obtained from Novabiochem (Merck, Darmstadt, Germany). Diethyl ether, Sodium acetate, tetrahydrofuran (THF), ethyl acetate, and acetonitrile were purchased from Spectrochem Pvt Ltd (Mumbai, India). Cell lines L-929 (Mouse fibroblast) from ATCC (Manassas, VA). Chloroquine resistant strains of P. falciparum (Indo) were obtained and grown in human O+ erythrocytes at 3 % hematocrit in a complete medium (RPMI 1640 medium supplemented with 25 mM HEPES, pH 7.5, 25 mM sodium bicarbonate, 50 mg/liter hypoxanthine,0.5 % Albumax II, and 40 µg/ml gentamicin sulfate). Cultures were maintained at 37 °C in a gas mixture of 5 % CO2 and 3 % O2. All other chemicals and buffers were of the highest grade available.
8
Quantification of Polyamines and Steroids
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Reference standards for nine polyamines and steroids were obtained from Sigma-Aldrich (St. Louis, MO, USA), Steraloids (Newport, RI, USA), and Tokyo Chemical Industry (Tokyo, Japan). The IS, d3-epitestosterone, and 1,6-diaminohexane were obtained from Sigma-Aldrich for calibration. For both calibration and QC, a commercially available blank serum sample (UTAK laboratories Inc, Valencia, CA, USA) was used. Sodium carbonate, sodium bicarbonate, formic acid (ACS reagent), and isobutyl chloroformate (derivatization solution) were obtained from Sigma-Aldrich. All high-performance liquid chromatography (HPLC)-grade organic solvents, including diethyl ether, acetonitrile, and methanol, were acquired from Burdick and Jackson (Muskegon, MI, USA). Deionized water (DW) was obtained using a Milli-Q purification system (Millipore, Billerica, MA, USA).
9
Comprehensive Chemical Reagent Acquisition
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Aluminium chloride (99%), ammonium bicarbonate (≥99.5%), L-aspartic acid disodium salt (≥98%), L-alanine (≥99.5), β-alanine (≥99%), calcium chloride dihydrate (≥99%), chloroform (100–200 ppm amylene stabiliser, ≥99.5), cobalt chloride hexahydrate (cell culture grade), glycine (≥99%), glutamate (≥99%), guanidine chloride solution (8 M), hydrochloric acid (≥37%), isobutyl chloroformate (98%), manganese sulfate (cell culture grade), oxaloacetic acid (≥97%), potassium chloride (99%), potassium phosphate monobasic (99.5–101.0%), pyridine (98%), pyridoxamine dihydrochloride (cell culture grade), pyridoxal hydrochloride (≥99%), L-serine (≥99%), sodium acetate (99–101%), sodium orthovanadate (99.98%), and zinc chloride x (≥98%) were purchased from Sigma. Boric acid (analytical grade), water (HPLC grade) and acetonitrile (HPLC grade) were purchased from Fischer. Copper sulfate hexahydrate (98.0–102.0%), isobutanol (99%), magnesium chloride hexahydrate (≥98%), and nickel chloride hexahydrate (98%) were from Alfa Aesar. Sodium hydroxide was purchased from VWR, and sodium chloride (research-grade) was purchased from Millipore. Ammonium chloride (analysis-grade) was from Acros and 9-fluroenylmethoxycarbonyl chloride (97%) was from Novabiochem. Helium (99.995%) and Nitrogen (99.998%) were purchased from BOC.
10
Immunoassay Development using Synthetic Standards
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TML, TML fumarate, and aminobutyric standards
were purchased from J&K Scientific Ltd. (Beijing, China). Keyhole
limpet hemocyanin (KLH; MW 4 500 000 Da), bovine serum
albumin (BSA; MW 67 000 Da), ovalbumin (OVA; MW 45 000
Da), complete Freund’s adjuvant, incomplete Freund’s
adjuvant, 3,3′,5,5′-tetramethylbenzidine (TMB), tributylamine,
isobutyl chloroformate, Tween-20, horseradish peroxidase, and gelatin
were obtained from Sigma Chemical Company (St. Louis, MO). Enzyme
immunoassay-grade horseradish peroxidase-labeled goat anti-mouse immunoglobulin
was purchased from Kang-cheng Bioengineering Co. (Shanghai, China).
Cell culture reagents, including poly(ethylene glycol) solution, HT
supplement, HAT supplement, and 1640 cell culture medium, were obtained
from Life Technologies Corporation (Shanghai, China).
Jieyi
Biotech. Co., Ltd. (Shanghai, China) provided all of the materials
used for creating test strips; these include glass fiber membrane
(Ahlstrom 8964) used for the sample pad, nitrocellulose (NC) membrane
used to immobilize the coating antigen, an H5076 filter paper for
the absorbent pad, and the backing material (poly(vinyl chloride),
PVC). Other reagents were of analytical grade and purchased from Sinopharm
Chemical Reagent Co., Ltd. (Shanghai, China)
were purchased from J&K Scientific Ltd. (Beijing, China). Keyhole
limpet hemocyanin (KLH; MW 4 500 000 Da), bovine serum
albumin (BSA; MW 67 000 Da), ovalbumin (OVA; MW 45 000
Da), complete Freund’s adjuvant, incomplete Freund’s
adjuvant, 3,3′,5,5′-tetramethylbenzidine (TMB), tributylamine,
isobutyl chloroformate, Tween-20, horseradish peroxidase, and gelatin
were obtained from Sigma Chemical Company (St. Louis, MO). Enzyme
immunoassay-grade horseradish peroxidase-labeled goat anti-mouse immunoglobulin
was purchased from Kang-cheng Bioengineering Co. (Shanghai, China).
Cell culture reagents, including poly(ethylene glycol) solution, HT
supplement, HAT supplement, and 1640 cell culture medium, were obtained
from Life Technologies Corporation (Shanghai, China).
Jieyi
Biotech. Co., Ltd. (Shanghai, China) provided all of the materials
used for creating test strips; these include glass fiber membrane
(Ahlstrom 8964) used for the sample pad, nitrocellulose (NC) membrane
used to immobilize the coating antigen, an H5076 filter paper for
the absorbent pad, and the backing material (poly(vinyl chloride),
PVC). Other reagents were of analytical grade and purchased from Sinopharm
Chemical Reagent Co., Ltd. (Shanghai, China)
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