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Flashsmart chns

Manufactured by Thermo Fisher Scientific
Sourced in United States

The FlashSmart CHNS is an elemental analyzer designed to determine the carbon, hydrogen, nitrogen, and sulfur content in a wide range of organic and inorganic samples. It utilizes combustion and detection techniques to provide rapid and accurate analysis of these elements.

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3 protocols using flashsmart chns

1

Elemental Composition Analysis of Samples

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The elemental analysis of the samples (the percentage of C, H and N; O was calculated by difference) was made using a Thermoscientific FlashSmart CHNS.
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2

Quantification of Functionalized Cellulose Nanocrystals

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The amount of carbon, nitrogen, oxygen, and sulfur in the CNC derivatives was quantified using a Thermo Scientific FlashSmart CHNS elemental analyser. The degree of substitution (DS) of CNC–N3 was directly calculated from the %N while the DS of CNC-DES was quantified from %C and calculated using (1) as previously reported [22 (link)]: DS=6×MC%C×MAGUMg×%CMCg where 6 x Mc is the carbon mass of one anhydroglucose unit (72.07 g/mol), MAGU is the mass of anhydroglucose unit (162.14 g/mol), Mg is the mass of the grafted moiety (404.4 g/mol), and MCg is the carbon mass of the grafted moiety (228.19 g/mol).
The degrees of surface substitution (DSsurf) were also derived from the DS values using (2): DSsurf=DS/Rc where Rc is the CNC chain ratio = 0.29 [23 (link)].
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3

Elemental Composition Analysis of Microalgae and Daphnia

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Before the beginning of the grazing experiment, samples of C. reinhardtii that had been grown in different conditions were collected for the analysis of cellular carbon, nitrogen, and phosphorus. Samples were taken from the respective culture bottles by filtering 15 to 25 mL of each culture onto pre-combusted (i.e., at 550 °C for 5 h) GF/C glass-fiber filters. After the seven-day grazing experiment or following 6-h starvation, five individuals of D. magna from each experimental group were transferred to a pre-combusted 25 μm GF/C filter for determination of elemental composition (C and N), and another five individuals of D. magna of similar body length and weight as the first five were collected for phosphorus measurement. Cellular carbon and nitrogen in both the D. magna and C. reinhardtii were measured with a CHNS (carbon, hydrogen, nitrogen, sulphur) elemental analyzer (FlashSmart CHNS, Thermo Scientific Inc. Massachusetts, USA) according to previously described protocol [78 (link)]. The amount of phosphorus (in the form of orthophosphate) was analyzed manually following acidic oxidative hydrolysis with 1% HCl [25 ] using a spectrophotometer at a wavelength of 880 nm, with a detection limit of 0.5 μmol/L.
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