Kyse450

Human ESCC cell lines including, KYSE30 (#0188) and KYSE450 (#1430) were from Japanese Collection of Research Bioresources (JCRB; NIBIO, Ibaraki, Osaka, Japan), and the normal esophagus epithelial cell line HET-1A (#2692) was from American Type Culture Collection (ATCC; Manassas, VA, USA). The KYSE30 cells were cultured in DMEM (Hyclone, Logan, UT, USA) with 2% fetal bovine serum (FBS; Hyclone), and KYSE30 cells were cultured in Ham’s F12 and RPMI1640 medium (1 to 1 mix; Hyclone) with 2% FBS, HET-1A cells were in DMEM plus 10% FBS. All the cells were incubated in a humidified atmosphere of 5% CO₂ at 37 °C.

A total of 21 ESCC cell lines, specifically KYSE30, KYSE70, KYSE140, KYSE150, KYSE180, KYSE270, KYSE410, KYSE450, KYSE510, KYSE590, KYSE890, KYSE1170, KYSE1260, KYSE1440, NUEC2, TE1, TE2, TE3, WSSC, TT, and TTn, along with Het‐1A, a non‐cancerous epithelial cell line, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), the American Type Culture Collection (Manassas, VA, USA), or were established at Nagoya University.
⁵ (link) The cells were cultured in a humidified incubator at 37°C with an atmosphere composition of 5% CO₂ using RPMI‐1640 (Sigma‐Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum and antibiotics.
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