Ifn γ pe 4s b3

Manufactured by BD

IFN-γ-PE (4S.B3) is a fluorescently-labeled antibody specific for interferon-gamma (IFN-γ). It is designed for use in flow cytometry applications to detect and quantify IFN-γ-producing cells. The antibody is conjugated to the R-Phycoerythrin (PE) fluorescent dye, which emits light in the yellow-orange spectrum when excited.

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Close spelling variants of this product

PE-conjugated anti-human IFN-γ (clone 4S.B3), IFN-γ (4S.B3) IFN-γ-PE IFN-γ

2 protocols using ifn γ pe 4s b3

Multiparameter Flow Cytometry Panels

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The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated (anti-human IL-22, RD, see reference(44 (link))), Streptavidin-Pacific blue (invitrogen). IL-4-APC (8D4-8, eBioscience), Perforin-biotinylated (Pf-344, Mabtech)

Yao S., Huang D., Chen C.Y., Halliday L., Wang R.C, & Chen Z.W. (2014). CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2120-2132.

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Comprehensive Immune Profiling by Flow Cytometry

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Flow cytometry analysis was performed as previously described (20 (link)). Single cells were stained with Alexa Fluor 594 dye (Thermo Fisher), to exclude dead cells from analysis, and then fixed in paraformaldehyde (PFA; 1.6%). Fixed cells were stained with the following antibodies: CD3-Pacific Blue (clone UCHT1), CCR7-PE (150503), CXCR5-Ax488 (RF8B2), CD20-APCH7 (L27), PDL1-APC (MIH1), PDL2-APC (MIH18), and IFNγ-PE (4S.B3) from BD Biosciences; TIGIT-APC (MBSA43), LAG3-PeCy7 (3DS223H), TNFα-Ax488 (MAb11), and IL2-PeCy7 (MQ1–17H12) from eBioscience; and CD4-Ax700 (RPA-T4), CD8-Bv785 (RPA-T8), CD45RA-Bv510 (HI100), PD1-Bv650 (EH12.2H7), TIM3-APC (F38–2E2), BTLA-APC (MIH26), CD244-PerCPCy5.5 (C1.7), CD160-PeCy7 (BY55), LAIR1-PerCPCy5.5 (NKTA255), CD155-PE (SKII.4), and CD112-PeCy7 (TX31) from BioLegend. Brilliant Stain Buffer (BD Biosciences) was used as staining buffer. Data were acquired on LSR II (BD Biosciences) and analyzed using Cytobank (https://www.cytobank.org/).

Josefsson S.E., Beiske K., Blaker Y.N., Førsund M.S., Holte H., Østenstad B., Kimby E., Köksal H., Wälchli S., Bai B., Smeland E.B., Levy R., Kolstad A., Huse K, & Myklebust J.H. (2019). TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma. Cancer immunology research, 7(3), 355-362.

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