Whole mount skeletons were stained with 0.05% alizarin red (A5533, Sigma-Aldrich) and 0.15% alcian blue 8GX (A5268, Sigma-Aldrich), and then transferred with glycerol/KOH solutions from 20% glycerol/1% KOH to 80% glycerol/1% KOH. For whole-mount lacZ staining, reporter mice were fixed in 1% formaldehyde and 0.2% glutaraldehyde in PBS for 90 min, stained in X-gal for 2 h at 37°C, and cleared in 80% glycerol/PBS. For histological analysis with alcian blue and von Kossa staining, sections were stained with 1% aqueous silver nitrate (196-00831, Fujifilm) solution and 1% alcian blue (pH 2.5, 8GX, Sigma-Aldrich) solutions. Unreacted silver was removed using 5% sodium thiosulfate. Counterstaining was performed using nuclear fast red staining. Micro-computed tomography (μCT) scanning of the harvested femurs was performed using a microfocus X-ray CT system SMX-90CT (Shimadzu, Kyoto, Japan); the three-dimensional construction software package TRI/3D-BON (Ratoc System Engineering, Tokyo) was used for quantitative analysis.
Microfocus x ray ct system smx 90ct
Manufactured by Shimadzu
Sourced in Japan
The Microfocus X-ray CT system SMX-90CT is a laboratory instrument designed for high-resolution computed tomography (CT) imaging. It utilizes X-ray technology to generate detailed three-dimensional (3D) images of various samples. The system is capable of analyzing the internal structure and composition of a wide range of materials, including metals, ceramics, and polymers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using microfocus x ray ct system smx 90ct
1
Skeletal Staining and Imaging Protocol
2
Skeletal Staining and Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole mount skeletons were stained with 0.05% alizarin red (A5533, Sigma-Aldrich) and 0.15% alcian blue 8GX (A5268, Sigma-Aldrich), and then transferred with glycerol/KOH solutions from 20% glycerol/1% KOH to 80% glycerol/1% KOH. For whole-mount lacZ staining, reporter mice were fixed in 1% formaldehyde and 0.2% glutaraldehyde in PBS for 90 min, stained in X-gal for 2 h at 37°C, and cleared in 80% glycerol/PBS. For histological analysis with alcian blue and von Kossa staining, sections were stained with 1% aqueous silver nitrate (196-00831, Fujifilm) solution and 1% alcian blue (pH 2.5, 8GX, Sigma-Aldrich) solutions. Unreacted silver was removed using 5% sodium thiosulfate. Counterstaining was performed using nuclear fast red staining. Micro-computed tomography (μCT) scanning of the harvested femurs was performed using a microfocus X-ray CT system SMX-90CT (Shimadzu, Kyoto, Japan); the three-dimensional construction software package TRI/3D-BON (Ratoc System Engineering, Tokyo) was used for quantitative analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!