Cd90 5e10 pe

Manufactured by BioLegend

Sourced in United States

CD90 (5E10) PE is a fluorescently-labeled monoclonal antibody that specifically binds to the CD90 (Thy-1) cell surface antigen. CD90 is a glycosylphosphatidylinositol-anchored glycoprotein that is expressed on various cell types, including T cells, hematopoietic stem cells, and fibroblasts. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD90-positive cells using flow cytometry.

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Lab products found in correlation

Facscalibur cytometer, by BD (1 mentions) Live dead fixable far red dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Facscalibur flow cytometer, by BD (1 mentions)

Close spelling variants of this product

CD90 PE-Cy7 (clone 5E10, Anti-CD90-PE Cy7 (clone 5E10, CD90-PE

2 protocols using cd90 5e10 pe

Immunophenotyping of Expanded Hematopoietic Stem and Progenitor Cells

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The immunophenotype of isolated and expanded HSPC was analyzed by flow cytometry. Briefly, cells were washed with PBS and viability was evaluated using a Far Red Fixable Dead Cell Stain Kit (Thermo Fisher Scientific). Afterward, cells were surface stained using previously titrated CD45RA (HI100) FITC (BD Biosciences), CD90 (5E10) PE (BioLegend) and CD34 (8G12) PerCP-Cy5.5 (BD Biosciences). Stained cells were acquired on a FACSCalibur™ cytometer (BD Biosciences). Data was analyzed using FlowJo v10 software (FlowJo LLC, Ashland, OR, USA).

Branco A., Tiago A.L., Laranjeira P., Carreira M.C., Milhano J.C., dos Santos F., Cabral J.M., Paiva A., da Silva C.L, & Fernandes-Platzgummer A. (2022). Hypothermic Preservation of Adipose-Derived Mesenchymal Stromal Cells as a Viable Solution for the Storage and Distribution of Cell Therapy Products. Bioengineering, 9(12), 805.

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HSPC Viability and Surface Markers

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HSPC before and after expansion (days 0 and 7, respectively) were firstly incubated with Far Red LIVE/DEAD Fixable Dead Cell Stain Kit (Thermo Fisher Scientific) to assess cell viability and then surface stained with the following anti-human antibodies: CD34 (8G12) PerCP-Cy5.5, CD41α (HIP8) PE (BD Pharmingen, USA); CD90 (5E10) PE, CD7 (CD7-6B7) FITC, CD14 (M5E2) FITC, CD15 (HI98) PE, and CD33 (WM53) PE (BioLegend, USA). Cells were acquired on a FACSCalibur flow cytometer (BD Biosciences, USA), and data was analyzed using FlowJo v10 software (FlowJo LLC, USA).

Bucar S., Branco A.D., Mata M.F., Milhano J.C., Caramalho Í., Cabral J.M., Fernandes-Platzgummer A, & da Silva C.L. (2021). Influence of the mesenchymal stromal cell source on the hematopoietic supportive capacity of umbilical cord blood-derived CD34+-enriched cells. Stem Cell Research & Therapy, 12, 399.

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