Bs 180 automatic biochemical analyzer

Non-anticoagulant blood was centrifuged (6000× g, 10 min, 25 °C) to obtain blood serum and then stored at −80 °C. Here, seven biochemical parameters related to metabolism were measured, which included the enzymatic activities of lactate dehydrogenase (LDH), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and the concentration of glucose (GLU), total cholesterol (TC), triglyceride (TG), and albumin (ALB). According to Ni et al., 2016, we used a BS-180 automatic biochemical analyzer (Mindray, China) to collect these data and strictly followed the instructions of related kits (Mindray, China) [52 (link)].

Serum metabolites were measured using a Mindray BS-180 Automatic Biochemical Analyzer (Mindray, Shenzhen, China) (n = 7-10 per group).

In each group, blood samples were collected from the external iliac artery and the pulmonary artery. Arterial and venous blood samples were used to measure blood gas parameters (GEM3000 Blood Gas Analyzer, Instrumentation Laboratory, America.), including arterial oxygen saturation (SaO2), venous oxygen saturation (SvO2), and lactate. DO₂ and VO₂ were calculated according to the following equations:
${\text{DO}}_2=1.34\times \text{SaO}2\times \text{Hemoglobin}\left(\text{g}/\text{L}\right)\times \text{CO}\times 10$
${\text{VO}}_2=\left(\text{SaO}2—\text{SvO}2\right)/\text{SaO}2$
Blood samples were also used to measure blood urea nitrogen, creatinine, alanine aminotransferase, and aspartate aminotransferase (AST) (BS-180 Automatic biochemical analyzer, Mindray Biomedical Electronics Co., Ltd, Shenzhen, China).