Freshly isolated cells were seeded on collagen IV-coated plates for 24—36 h to revive CD44 expression diminished by digestive enzymes. Both unattached and attached (trypsinized) cells were collected and blocked with 1% BSA, 4% normal goat serum in PBS. Cells were stained with AF488-EpCAM (BioLegend), APC anti-human CD44 (BioLegend), and their respective isotypes. Cells were sorted using a Beckman Coulter MoFlo high-speed cell sorter. Unstained, isotype, and single-color controls were performed. The gates for CD44 and EpCAM were set based on the results of isotype, and single-color controls were run in parallel. The results were analyzed using FlowJo 10.1.
Af488 epcam
Manufactured by BioLegend
AF488-EpCAM is a fluorochrome-conjugated antibody that binds to the Epithelial Cell Adhesion Molecule (EpCAM) protein. It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using af488 epcam
1
CD44 and EpCAM Expression Profiling
2
Isolation and Characterization of CD44-Expressing Cells
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Freshly isolated cells were seeded on collagen IV coated plates for 24 - 36 h to revive CD44 expression diminished by digestive enzymes. Both unattached and attached (trypsinized) cells were collected and blocked with 1% BSA, 4% normal goat serum in PBS. Cells were stained with AF488-EpCAM (BioLegend), APC anti-human CD44 (BioLegend), and their respective isotypes. Cells were sorted using a Beckman Coulter MoFlo high-speed cell sorter. Unstained, isotype and single-color controls were performed. The gates for CD44 and EpCAM were set based on the results of isotype, and single-color controls were run in parallel. The results were analyzed using FlowJo 10.1.
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