C1600

Blood was drawn from the subjects fasting for 8–12 h,a random spot urine sample was collected. Fasting blood glucose (FBG), lipid profile, blood urea nitrogen (BUN), serum creatinine (Scr), calcium, phosphate, and HbA1c were among the laboratory markers. Using an automated analyzer called the Abbott C-1600, blood biochemical indicators were looked at.Using the glucose oxidase technique, FBG was found.Urinary albumin and creatinine concentrations were measured using a turbidimetric immunoassay with a Hitachi-7600 analyzer. Albuminuria was defined according to the creatinine,which was calculated by dividing the urinary albumin concentration (mg) by the urinary creatinine concentration (mg), which was urinary albumin-to-creatinine ratio (UACR).We determined serum 25(OH)D concentrations using using immunoassay chemiluminescence detection on Abbott ISR-2000 automatic analyzer.HbA1c was measured by ionic exchange high-performance liquid chromatography. Data support was provided by the Laboratory Department of The People's Hospital of Gansu Province.

Clinical data from the patients were collected twice: at pre- and post-Cana treatment. Meanwhile, data from the healthy controls were obtained at baseline. Demographic and health-related data such as name, gender, age, medication history, surgical history, history of contact lens use, past medical conditions, and dietary habits were collected using comprehensive questionnaires. Height and weight measurements were taken in the morning on an empty stomach, and all participants wore uniform clothing. FPG concentrations were measured using an Automatic Biochemical Analyzer (TBA-120 FR, Toshiba, Japan). Glycated serum protein (GSP) levels were measured using an automatic biochemical analyzer (ADVIA Chemistry XPT, Siemens, USA). Plasma HbAlc concentrations were measured using high-performance liquid chromatography (Bio-Rad D-10, Bio-Rad Laboratories Co., Ltd., Germany). Routine blood tests were performed using the Swelab Alfa Cell analyzer (Boule Diagnostics, AB, Sweden). Urinary microalbumin (UMALB) and creatinine (UCR) levels were assessed using a DCA Vantage Analyzer (Siemens, Chapel Lane Swords Co., Dublin, Ireland). Triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels were measured using an automatic biochemical analyzer (Abbott C1600, Illinois, USA).

After the water maze experiment, the rats were fasted for 12 hours but were allowed to drink water freely. After the rats were anesthetized, blood was taken from the abdominal aorta, and the collected blood was centrifuged to obtain serum. The content of T3 and T4 in serum was measured by an automatic biochemical analyzer (C1600, Abbott, USA) within 48 hours. The rats were euthanized with an overdose of sodium pentobarbital, the brain tissues of the rats were separated, a part of the brain tissue was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and the rest was stored in a refrigerator at − 80 °C for Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR).