Dl apv

Manufactured by R&D Systems

Sourced in United States, United Kingdom

The DL-APV is a laboratory instrument designed for the analysis and purification of protein samples. It utilizes an affinity-based chromatography technique to separate and purify target proteins from complex mixtures. The DL-APV is capable of handling a wide range of sample sizes and can be used in various research and development applications.

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Lab products found in correlation

Qx314 cl, by R&D Systems (3 mentions) Kynurenic acid, by R&D Systems (3 mentions) Clozapine n oxide, by Enzo Life Sciences (1 mentions) Methadone hydrochloride, by Merck Group (1 mentions) Biocytin, by Merck Group (1 mentions) Sr95531, by Merck Group (1 mentions) Zd7288 hydrate, by Merck Group (1 mentions) Tetrodotoxin, by Merck Group (1 mentions)

7 protocols using dl apv

Obtaining Reagents for Experiments

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DL-APV was obtained from R&D Systems (Minneapolis, MN). Clozapine N-oxide (CNO) was obtained from Enzo Life Sciences (Pittsburgh, PA). All other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO).

Yi F., DeCan E., Stoll K., Marceau E., Deisseroth K, & Lawrence J.J. (2014). Muscarinic excitation of parvalbumin-positive interneurons contributes to the severity of pilocarpine-induced seizures. Epilepsia, 56(2), 297-309.

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Neurochemicals for Brain Research

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Chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Kynurenic acid, DL-APV, and QX314-Cl were purchased from Tocris/R&D Systems (Bristol, UK). Methadone hydrochloride was acquired through the NIH Drug Supply Program and purchased from Sigma-Aldrich.

Gamble M.E., Marfatia R, & Diaz M.R. (2022). Prenatal methadone exposure leads to long-term memory impairments and disruptions of dentate granule cell function in a sex-dependent manner. Addiction biology, 27(5).

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Neurochemicals for Electrophysiology

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DL-APV was purchased from R&D Systems (Minneapolis, MN, USA). Tetrodotoxin (cat# 5651), TEA (cat# 2265), 4-AP (cat# A78403), DNQX (cat# D0540), SR-95531 (cat# S106), and ZD7288 hydrate (cat# Z3777) were purchased from Sigma-Aldrich, Inc. (Saint Louis, MO, USA). Salts and chemicals for saline solutions, including biocytin, were also purchased from Sigma-Aldrich, Inc.

Sekulić V., Yi F., Garrett T., Guet-McCreight A., Lawrence J.J, & Skinner F.K. (2020). Integration of Within-Cell Experimental Data With Multi-Compartmental Modeling Predicts H-Channel Densities and Distributions in Hippocampal OLM Cells. Frontiers in Cellular Neuroscience, 14, 277.

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Sourcing Organic Compounds for Research

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless noted. Kynurenic acid, DL-APV, and QX314-Cl were purchased from Tocris/R&D Systems (Bristol, UK). Chemicals purchased from other vendors are indicated below.

Varlinskaya E.I., Johnson J.M., Przybysz K.R., Deak T, & Diaz M.R. (2019). Adolescent forced swim stress increases social anxiety-like behaviors and alters kappa opioid receptor function in the basolateral amygdala of male rats. Progress in neuro-psychopharmacology & biological psychiatry, 98, 109812.

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Purchasing Chemicals for Neuroscience Research

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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Kynurenic acid, DL-APV, and QX314-Cl were purchased from Tocris/R&D Systems (Bristol, UK).

Przybysz K.R., Gamble M.E, & Diaz M.R. (2021). Moderate adolescent chronic intermittent ethanol exposure sex-dependently disrupts synaptic transmission and kappa opioid receptor function in the basolateral amygdala of adult rats. Neuropharmacology, 188, 108512.

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Neurotransmitter regulation assay

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Acetylcholine chloride (A6625), DNQX (D0540), and gabazine (SR-95531; S106) were obtained from Sigma-Aldrich Inc. (St. Louis, MO). DL-APV was obtained from R&D Systems (Minneapolis, MN).

Yi F., Catudio-Garrett E., Gábriel R., Wilhelm M., Erdelyi F., Szabo G., Deisseroth K, & Lawrence J. (2015). Hippocampal “cholinergic interneurons” visualized with the choline acetyltransferase promoter: anatomical distribution, intrinsic membrane properties, neurochemical characteristics, and capacity for cholinergic modulation. Frontiers in Synaptic Neuroscience, 7, 4.

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Reagents for Cell Culture Protocols

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DL-APV was obtained from R&D (R&D Systems, Minneapolis, MN). All other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO).

Yi F., Garrett T., Deisseroth K., Haario H., Stone E, & Lawrence J.J. (2021). Septohippocampal transmission from parvalbumin-positive neurons features rapid recovery from synaptic depression. Scientific Reports, 11, 2117.

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