Antibodies against β actin and flag

The COX-1 inhibitor (Sc-560) and an enzyme-linked immunosorbent assay (ELISA) kit for porcine PGE2 were purchased from Cayman systems. The COX-2 inhibitor (celecoxib) and the 14-3-3 inhibitor (BV02) were from Sigma. NF-κB inhibitor (BAY11-7082), p38MAPK inhibitor (SB203580), PKC inhibitor (GF-109203X), JNK inhibitor (SP600125) and MEK1 inhibitor (AZD6244) were from Selleck. All inhibitors were reconstituted in dimethyl sulfoxide (DMSO), and DMSO was used as the solvent control for all experiments involving treatment with inhibitors. TRIzol was from Invitrogen Life Technology. The Dual-Glo luciferase assay system was from Promega.
Antibodies against COX-1, ERK, p-ERK, p-C/EBPβ, and 14-3-3ζ were purchased from Cell Signaling Technology. Anti-COX-2 antibody was from Merk. And antibodies against β-actin and Flag were obtained from Sigma. Anti-HA antibody was from Santa Cruz, and anti-C/EBPβ antibody was purchased from GeneTex. Anti-NSP2 antibody was a gift from Dr. Chang-jiang Weng (Harbin Veterinary Research Institute, CAAS, China).

SuperScript™III, RPMI 1640 medium and Opti-MEM medium were obtained from Invitrogen (Carlsbad, CA). TriSolution Plus Reagent was from GeneMark (Atlanta, Georgia). RNAzol^® RT RNA isolation reagent was purchased from Molecular Research Center, INC (Cincinnati, OH). GoScript^TM Reverse Transcriptase, Go Taq^® Green Master Mix (2X), and luciferase assay system were from Promaga (Madison, WI). G418 disulfate salt was from Sigma-Aldrich (St. Louis, MO). Src inhibitor PP1 was from Calbiochem (Merck Millipore, Darmstadt, Germany). Src inhibitor-1 was from Merck Millipore (Burlington, MA, USA). HyFect^TM DNA transfection reagent was from Leadgene Biomedical (Tainan, Taiwan). Antibodies against β-actin and Flag were obtained from Sigma-Aldrich (St. Louis, MO). E-cadherin and N-cadherin antibodies were obtained from BD Bioscience (Bedford, MA). MMP-2 antibody was purchased from Millipore (Billerica, MA). Antibodies against CDCP1, c-Src, pSrc^Tyr416, and pPKCδ^Tyr314 were purchased from Cell Signaling Technology (Danvers, MA).