PAC, ATRA, BMS753, LG100268, BMS195614, and verapamil were purchased from Sigma-Aldrich (St. Louis, MO, USA). UVI3003, 3,5-bis(trifluoro-methyl)pyrazole (BTP-2), and NSC-95397 and antibodies for phospho-MAPK kinase (p-MEK)-1/2, p-extracellular signal-regulated kinase- (pERK-) 1/2, ERK-1/2, p53, cyclin B1, caspase-3, and β-actin were supplied by Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies for caspase-8 and caspase-9 were procured from Cell Signaling Technology (Danvers, MA, USA).
Bms195614
Manufactured by Merck Group
Sourced in United States
BMS195614 is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Lg100268, by Merck Group (4 mentions)
Bms753, by Merck Group (4 mentions)
Uvi3003, by Santa Cruz Biotechnology (2 mentions)
Nsc 95397, by Santa Cruz Biotechnology (1 mentions)
3 5 bis trifluoromethyl pyrazole btp2, by Santa Cruz Biotechnology (1 mentions)
Cyclin b1, by Santa Cruz Biotechnology (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
Verapamil, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Uvi3003, by Merck Group (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Bis sulfosuccinimidyl suberate bs3, by Thermo Fisher Scientific (1 mentions)
9cis retinoic acid 9cisra, by Merck Group (1 mentions)
Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
4 protocols using bms195614
1
Molecular Mechanisms of Apoptosis Regulation
2
Retinoid Receptor Agonists and Antagonists
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ATRA, RARα agonist BMS753, RARα antagonist BMS195614, retinoid X receptor (RXR) α agonist LG100268, and pan RXR antagonist UVI3003 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibiotic-antimycotic, HEPES (4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid) buffer, and β-mercaptoethanol were purchased from Invitrogen Corp (Gibco BRL, MD, USA).
3
Regulation of Cellular Signaling Pathways
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The following chemicals were used: ATRA, RAR α agonist (BMS753), RXR α agonist (LG100268), RARα antagonist (BMS195614), GA, EGS, lipid raft inhibitor filipin III (F-4767), caveolin inhibitor methyl-beta-cyclodextrin (C-4555) from Sigma-Aldrich (St. Louis, MO, USA); bis (sulfo-succinimidyl) suberate (BS3) from Thermo Fisher Scientific (Rockford, IL, USA); RXRα antagonist (UVI 3003) from Santa Cruz Biotechnology (Dallas, TX, USA); flagellin from S. typhimurium, GeB from InvivoGen (San Diego, CA, USA); 17-AAG and radicicol from Cayman Chemical (Ann Arbor, MI, USA); dynamin inhibitor dynasore (ab120192), Dyngo® 4a (ab120689) from Abcam (Cambridge, UK).
4
Retinoid Signaling Pathway Modulation
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ATRA, RARα agonist BMS753, RXRα agonist LG-100268, RARα antagonist BMS195614, 9-cis-retinoic acid (9cis-RA), and dexamethasone were obtained from Sigma-Aldrich (St. Louis, MO, USA). RXRα antagonist UVI3003, anti-β-actin antibody, and secondary antibodies for western blot were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). RPMI-1640 medium for cell culture was obtained from Welgene Inc. (Gyongsan, Korea). Fetal bovine serum (FBS) was purchased from Invitrogen (Gibco BRL, MD, USA).
ATRA was prepared as a 20 mM stock in dimethyl sulfoxide (DMSO) and stored at -20℃ before being diluted to the desired concentration in medium in each experiment.
Purified anti-CPD antibody (ab184960) used in flow cytometric analysis was purchased from Abcam (Cambridge, MA, USA).
ATRA was prepared as a 20 mM stock in dimethyl sulfoxide (DMSO) and stored at -20℃ before being diluted to the desired concentration in medium in each experiment.
Purified anti-CPD antibody (ab184960) used in flow cytometric analysis was purchased from Abcam (Cambridge, MA, USA).
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