Sodium alginate was purchased from Junsei Chemical Co. (Tokyo, Japan). A live/dead cell viability kit was purchased from Invitrogen (Carlsbad, CA, USA). Tartrazine was purchased from GreenTech (Daejeon, South Korea). Horse serum and fungizone were purchased from Gibco (Grand Island, NY, USA). Dulbecco’s Modified Eagle’s medium (DMEM), penicillin-streptomycin (P/S), fetal bovine serum (FBS), L -glutamine, and phosphate-buffered saline (PBS; pH 7.4) were purchased from WelGene (Daegu, Gyeongbuk, South Korea). Zein powder, calcium chloride dihydrate, AESO, PEGDA (molecular weight: 575 g/mol), lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, ≥95%), collagenase I, entactin-collagen-laminin (ECL), insulin, linoleic acid, Nile red, bovine serum albumin, and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals used in this study were of analytical grade.
Sodium alginate
Manufactured by Junsei
Sourced in Japan
Sodium alginate is a naturally-derived polysaccharide extracted from brown seaweed. It is a versatile compound used in various laboratory applications, primarily as a thickening, stabilizing, and gelling agent.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Samchun (2 mentions)
Hydrochloric acid (hcl), by Samchun (2 mentions)
Fungizone, by Thermo Fisher Scientific (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Live dead cell viability kit, by Thermo Fisher Scientific (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Zein powder, by Merck Group (1 mentions)
Lithium phenyl 2 4 6 trimethylbenzoylphosphinate lap 95, by Merck Group (1 mentions)
Nile red, by Merck Group (1 mentions)
Collagenase 1, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Pegda, by Merck Group (1 mentions)
Chitosan, by Carl Roth (1 mentions)
Glycerol, by Daejung Chemicals (1 mentions)
Cacl2, by Samchun (1 mentions)
Fecl3 6h2o, by Samchun (1 mentions)
Nabh4, by Samchun (1 mentions)
Potassium iodide, by Samchun (1 mentions)
6 protocols using sodium alginate
1
Fabrication of Biomimetic Cell Scaffolds
2
Alginate-Calcium Lactate Bead Synthesis
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Sodium alginate (Junsei Chemical Co., Ltd., Tokyo, Japan) and calcium lactate (Daejung chemical & metal Co., Ltd., Siheung, Korea) were used as the functional materials for CAG beads. All chemicals and reagents used in this study were of an analytical grade.
3
Fabrication of Polysaccharide-Based Hydrogels
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sodium alginate and κ-carrageenan were dissolved in distilled water,
containing a co-polymer and/or crosslinkers. Hydrogel 1 was prepared as follows:
5% sodium alginate (Junsei chemical, Tokyo, Japan) and 1% chitosan (Carl Roth
GmbH, Karlsruhe, Germany) were dissolved in 40 mL of glycerol (Daejung, Siheung,
Korea). About 0.2% of CaCl2 (Samchum chemical, Pyeongtaek, Korea) was
dissolved in 60 mL of water (H2O), and this solution was added to the
glycerol mixture to provide Ca2+ ion. After stirring for 20 min, this
mixture was poured into a flat square-shaped dish (SPL life science, Pocheon,
Korea) and stored at 42℃ to dry the hydrogel for 2 days (Lee et al., 2003 (link)). Hydrogels 2, 3, and 4
were prepared as follows: 1% (hydrogel 2) or 2% of κ-carrageenan
(hydrogels 3 and 4) in 100 mL of H2O were stirred on a heat block at
80℃ (Mohamadnia et al., 2008 (link)),
followed by the addition of 1% chitosan (hydrogel 2), 1% CaCl2(hydrogel 3), or 3% CaCl2 (hydrogel 4). The solutions were poured
into the flat square-shaped plate, which were placed at room temperature
(25℃) to form hydrogels.
containing a co-polymer and/or crosslinkers. Hydrogel 1 was prepared as follows:
5% sodium alginate (Junsei chemical, Tokyo, Japan) and 1% chitosan (Carl Roth
GmbH, Karlsruhe, Germany) were dissolved in 40 mL of glycerol (Daejung, Siheung,
Korea). About 0.2% of CaCl2 (Samchum chemical, Pyeongtaek, Korea) was
dissolved in 60 mL of water (H2O), and this solution was added to the
glycerol mixture to provide Ca2+ ion. After stirring for 20 min, this
mixture was poured into a flat square-shaped dish (SPL life science, Pocheon,
Korea) and stored at 42℃ to dry the hydrogel for 2 days (Lee et al., 2003 (link)). Hydrogels 2, 3, and 4
were prepared as follows: 1% (hydrogel 2) or 2% of κ-carrageenan
(hydrogels 3 and 4) in 100 mL of H2O were stirred on a heat block at
80℃ (Mohamadnia et al., 2008 (link)),
followed by the addition of 1% chitosan (hydrogel 2), 1% CaCl2(hydrogel 3), or 3% CaCl2 (hydrogel 4). The solutions were poured
into the flat square-shaped plate, which were placed at room temperature
(25℃) to form hydrogels.
4
Synthesis and Characterization of Heavy Metal Adsorbents
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The reagent FeCl3.6H2O (97% purity), NaBH4 (98% purity), Carboxymethyl cellulose sodium salt, CaCl2 (96% purity), NaOH, and HCl were procured from “Samchun Pure Chemical Co. Ltd, Korea” and Ni(NO3)2.6H2O (97% purity) and Sodium Alginate were supplied by “Junsei Chemical Co., Ltd., Japan”. The commercial natural zeolite was purchased from Bear River Zeolite, a United States Antimony Corporation division. 1000 mg/L of Cu(II) and Cr(VI) solutions were prepared from Cu(NO3)2. 3H2O (99.5% purity) and K2CrO4 were purchased from “Duksan pure chemical co., Ltd, South Korea” and “Junsei Chemical Co., Ltd., Japan”, respectively.
5
Synthesis and Characterization of Alginate-Calcium Hydrogels
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Sodium alginate (molecular weight: 220,000) and calcium lactate were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan) and Daejung Chemicals and Metals Co., Ltd. (Gyeonggi, Korea), respectively. Standard solutions for measuring sodium and calcium ion content were obtained from AccuStandard (1000 µg/mL in 2−5% nitric acid; Sodium ICP Standard, New Haven, CT, USA) and PerkinElmer (100 µg/mL in 5% HNO3; Quality Control Standard-21 Elements, MA, USA), respectively. All other chemicals and reagents used were of analytical grade.
6
Synthesis of Bismuth Nanoparticles
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Bismuth (III) nitrate pentahydrate (Bi(NO3)3·5H2O; 98%), potassium iodide (KI; 99.5%), sodium hydroxide (NaOH; 97%), hydrochloric acid (HCl; 35%), and ethanol (94.5%) were purchased from Samchun Chemicals Co., Ltd. (Seoul, Korea). Sodium alginate was obtained from Junsei Chemical Co., Ltd. (Tokyo, Japan). CaCl2 was obtained from Dongyang Chemical Co., Ltd. (Yeongam-gun, Korea). Ethylene glycol (C2H4(OH)2; 99.8%) was purchased from Sigma-Aldrich Co., LLC (St. Louis, MO, USA). All chemicals were used without purification. Ultrapure water (deionized water; DI) was produced using a water purification system (Synergy®, Merck, Kenilworth, NJ, USA).
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