All reagents were from Sigma-Aldrich or Life Technologies (including Invitrogen), unless otherwise stated. A previously reported16 (link),54 (link) human GPR37L1 was purchased from Multispan; however, this construct lacked the endogenous GPR37L1 signal peptide and instead had an optimized signal peptide and N-terminal Flag tag, so we reintroduced the original sequence with a Hind III restriction site and Kozak sequence by polymerase chain reaction. All constructs in pcDNA5/FRT/TO or pcDNA3 contain human GPR37L1 with eYFP fused to its C-terminus; human GPR37L1-eYFP (wild type and Δ122) was cloned into p426GPD for S. cerevisiae experiments. Constructs were verified by DNA sequencing. Epitope tagging of GPR37L1 at the N-terminus and expression in Flp-In HEK293 cells resulted in the appearance of large vacuoles and subsequent cell death. N-terminal CSF and “tethered” peptides were synthesized by GenScript. Chimeric G protein yeast strains were provided by GSK under material transfer agreement.
N terminal csf and tethered peptides
Manufactured by GenScript
N-terminal CSF and 'tethered' peptides are laboratory equipment used for peptide synthesis and modification. They facilitate the creation of peptides with customized N-terminal regions and covalent linkages between peptides. The core function of these products is to enable precise control over the structure and properties of synthesized peptides, without interpretation or extrapolation on their intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n terminal csf and tethered peptides
1
Reintroduction of GPR37L1 Signal Peptide
2
Optimized GPR37L1 Expression Construct
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were from Sigma-Aldrich or Life Technologies (including Invitrogen), unless otherwise stated. A previously reported (16, 54) human GPR37L1 was purchased from Multispan; however, this construct lacked the endogenous GPR37L1 signal peptide and instead had an optimized signal peptide and N-terminal Flag tag, so we reintroduced the original sequence with a Hind III restriction site and Kozak sequence by polymerase chain reaction. All constructs in pcDNA5/FRT/TO or pcDNA3 contain human GPR37L1 with eYFP fused to its C-terminus; human GPR37L1-eYFP (wild type and Δ122) was cloned into p426GPD for S. cerevisiae experiments. Constructs were verified by DNA sequencing. Epitope tagging of GPR37L1 at the N-terminus and expression in Flp-In HEK293 cells resulted in the appearance of large vacuoles and subsequent cell death. N-terminal CSF and "tethered" peptides were synthesized by GenScript. Chimeric G protein yeast strains were provided by GSK under material transfer agreement.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!