Nev10020ex

OsteoSense™ dye (OsteoSense 680 EX and NEV10020EX; PerkinElmer, MA, USA) was reconstituted in DPBS in a concentration of 20 nmol/ml. We anesthetized the mice with isoflurane inhalation and injected the dye in a dose of 2 nmol/20 g body weight through the retro-orbital venous sinus. Imaging was performed 24 h post-injection. We euthanized the mice with CO₂ inhalation, perfused with 5 ml of PBS, and analyzed the isolated hearts ex vivo by an IVIS Spectrum In Vivo Imaging System (PerkinElmer, MA, USA).

OsteoSense dye (OsteoSense 680 EX and NEV10020EX; PerkinElmer, MA, United States) and near-infrared imaging were used to evaluate aorta calcification in mice as previously described (Malhotra et al., 2019 (link)). Mice (Ctrl, CKD, and CKD + DPD; n = 5/group) were anesthetized with isoflurane and injected with 2 nmol OsteoSense dye dissolved in 100 µL DPBS retro-orbitally. After 24 hours, mice were killed by CO₂ inhalation; the mice were perfused with 5 ml of ice-cold PBS, and aortas were isolated, cleaned, and analyzed ex vivo by an IVIS Spectrum In Vivo Imaging System (PerkinElmer, MA, United States). The observer who performed this measurement was blinded to the group assignment.