to 0.3% or to 0.03% w/v in Milli-Q water, or in the following buffers:
10 mM acetate Tris, pH 3.5; 10 mM MES, pH 5.0; 10 mM Tris HCl, pH
7.0; 10 mM CAPSO, pH 9.8. The photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate
(LAP, Sigma-Aldrich) was added at the final concentration of 0.2%
or 0.04% v/w and photopolymerized for 10 min under UV light, λ
= 365 nm (UView Mini Transilluminator, BioRad, Hercules, US). Samples
were prepared at least in triplicate.