LPS and Griess reagent (modified) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse TNF-α ELISA kit and IL-6 ELISA kit were purchased from eBioscience (San Diego, CA, USA). The antibody COX2, iNOS, phos-P65 (Ser536), P65, phos-TBK1, TBK1, phos-AKT (Ser473), AKT, phos-S6K (Thr389), S6K, phos–P38 (Thr180/Tyr182), P38, phos–JNK(Thr183/Tyr185), JNK, GAPDH (Cell Signaling, Danvers, MA, USA) and phos–ERK (Tyr204), ERK (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), HRP-linked anti-mouse and anti-rabbit antibodies were obtained from Santa Cruz. SYBR Select Master Mix for RT-PCR amplification was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). Primers for TNF-α, IL-6, iNOS and GAPDH were from Invitrogen Life Technologies (Carlsbad, CA, USA).
Phos s6k
Manufactured by Cell Signaling Technology
Sourced in United States
Phos-S6K is a primary antibody that recognizes phosphorylated ribosomal protein S6 kinase (S6K) at specific serine residues. S6K is a key regulator of cell growth and proliferation, and its phosphorylation status is an important indicator of the activation of the mTOR signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Cox 2, by Cell Signaling Technology (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Modified griess reagent, by Merck Group (1 mentions)
Mouse tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Il 6 elisa kit, by Thermo Fisher Scientific (1 mentions)
Phos p65, by Cell Signaling Technology (1 mentions)
Phos akt, by Cell Signaling Technology (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Nitrocellulose membrane, by Cytiva (1 mentions)
Anti lc3, by Cell Signaling Technology (1 mentions)
Phos rps6, by Cell Signaling Technology (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Beclin 1, by Abcam (1 mentions)
2 protocols using phos s6k
1
Investigating Inflammatory Signaling Pathways
2
Western Blot Analysis of Autophagy Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Embryos were deyolked80 (link), and then homogenized in lysis buffer (20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM EDTA, 10% glycerol, 0.1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor (Roche)). Protein lysates were separated on SDS-PAGE, transferred to nitrocellulose membrane (Amersham Biosciences), and incubated with anti-LC3 (Cell Signaling Technology, 1:1 000), phos-S6K (Cell Signaling Technology, 1:1 000), phos-RPS6 (Cell Signaling Technology, 1:1 000), phos-elf2a (Cell Signaling Technology, 1:1 000), p62 (MBL,1:1 000), Beclin1 (abcam, 1:5 000), p53 (a gift from Jinrong Peng, Zhejiang University) or anti-α-tubulin (Sigma, 1:2 000) antibodies, and then HRP-conjugated secondary Antibodies.
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