The contents of artemisinin in QHP were determined by HPLC. Quantitative analysis of artemisinin in QHP was performed on the Agilent 1290 Infinity apparatus comprising two solvent delivery systems and a photodiode array detector (Agilent, USA). The column was an Agilent ZORBAX SB-C18 chromatographic column (4.6 mm × 250 mm, 5.0 μm). The mobile phase consisted of acetonitrile and H2O (60: 40), and the pH value was 6.8–7.2. Reagents were filtered through a Millipore 0.45 mm filter and degassed prior to use. The entire run was carried out by gradient elution at a flow rate of 1.0 mL/min; the detection wavelength was set at 210 nm; the column was maintained at 35°C, and the injection volume was 10 μL. Data collection and quantification were performed with Agilent Open LAB A.02.02 CDS ChemStation (Agilent, USA). The peak of artemisinin was identified by comparison with chemical standards.
Zorbax sb c18 chromatographic column
Manufactured by Agilent Technologies
Sourced in United States
The Zorbax SB-C18 is a reverse-phase chromatographic column manufactured by Agilent Technologies. It is designed for the separation and analysis of a wide range of organic compounds in liquid chromatography (LC) applications.
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Lab products found in correlation
Agilent 1100 series, by Agilent Technologies (4 mentions)
Ion trap sl, by Brucker (3 mentions)
1290 infinity, by Agilent Technologies (1 mentions)
0.45 mm filter, by Merck Group (1 mentions)
Agilent open lab a 02.02 cds chemstation, by Agilent Technologies (1 mentions)
Agilent 1260 series hplc system, by Agilent Technologies (1 mentions)
1260 series hplc, by Agilent Technologies (1 mentions)
Centrivap, by Labconco (1 mentions)
Milli q rg system, by Merck Group (1 mentions)
Ph meter, by Mettler Toledo (1 mentions)
Agilent 1220 infinity hplc system, by Agilent Technologies (1 mentions)
Mikro 220r, by Hettich (1 mentions)
Agilent 1200 chromatograph, by Agilent Technologies (1 mentions)
Agilent 1100 hplc, by Agilent Technologies (1 mentions)
15 protocols using zorbax sb c18 chromatographic column
1
Quantitative Analysis of Artemisinin in QHP
2
Quantitative Determination of Atomoxetine and Metabolite
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During both study periods 5 mL of venous blood was drawn into heparinized tubes before drug administration as well as at 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8, 10, 12, 24, 36 and 48 hours post- dose. The separated plasma was stored frozen (−20°C) until analysis. Atomoxetine and 4-hydroxyatomoxetine-O-glucuronide plasma concentrations were determined using a validated high-throughput liquid chromatography-mass spectrometry method. The HPLC system was an Agilent 1100 series (binary pump, autosampler, thermostat) (Agilent Technologies, USA) and was coupled with a Brucker Ion Trap SL (BruckerDaltonics GmbH, Germany). A Zorbax SB-C18 chromatographic column (100 mm x 3.0 mm i.d., 3.5 μm) (Agilent Technologies) was used. The mobile phase was a mixture of 2 mM ammonium formate solution and acetonitrile, elution in gradient: 11 % acetonitrile at start, 41% at 2 minutes. The flow rate was 1 mL/min and the thermostat temperature was set at 48°C. The mass spectrometry detection was in single ion monitoring mode, positive ions, using an electrospray ionization source. The ions monitored were m/z 256 for atomoxetine and m/z 448 for its metabolite, respectively. The retention times for atomoxetine and 4-hydroxyatomoxetine-O-glucuronide were 4.1 min and 2.2 min, respectively. The calibration curves for both atomoxetine and its metabolite were linear between 8–600 ng/mL.
3
Creatinine-Adjusted Urine BPA Quantification
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Both the free BPA and BPA-GLU values obtained in this study were corrected for creatinine. To assess the impact of creatinine adjustment on the total variance of spot urine samples, urine creatinine levels were analyzed using a modified method developed and validated for creatinine analysis by Park et al (17 (link)). Briefly, a 10 µL aliquot of urine was diluted with milli-Q water (1000-fold) and 100 µL (5 mg/L) of creatinine-d3 (internal standard, 5 mg/mL) was added. Creatinine was analyzed with LC-MS/MS in electrospray positive ionization mode and the SRM transitions monitored were 114.1>86.1 for creatinine and 117.2>89.2 for creatinine-d3. One microliter of the extract was injected onto an Agilent (Agilent Technologies, CA, USA) Zorbax SB-C18 chromatographic column (3 x 50 mm, 3.5 µm particle sizes). The mobile phases A (water) and B (methanol) both contained 2 mM ammonium acetate. The analysis for creatinine was achieved using isocratic conditions (80%B).
4
Electron Beam Irradiation for Mycotoxin Analysis
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Samples were irradiated at doses of 5–50 kGy using an industrial electron accelerator at room temperature (25 °C) in Wuxi EL PONT Radiation Technology Co., Ltd., China. The energy of accelerated electrons was 5 MeV, and the beam current was 20 mA with 1000 mm scan width and a dose rate of 2 kGy/s. An Agilent 1260 series HPLC system (Agilent Technologies, Palo Alto, CA, USA) with a fluorescence detector was used for mycotoxin analysis. An Agilent ZORBAX SB-C18 chromatographic column (150 mm × 4.6 mm; 5 µm particle size; Agilent Technologies) was utilized for separation.
5
HPLC Analysis of Organic Compounds
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The chromatographic conditions were as followes: Agilent Zorbax-SB C18 chromatographic column (250 mm × 4.6 mm, 5 μm), the mobile phase consisted of 0.1% phosphoric acid aqueous solution (A) - acetonitrile (C) with a gradient elution: 0–5 min, 30% C; 5–8 min, 35% C; 8–13 min, 40% C; 13 min, 45% C; flow rate: 0.8 mL/min; detection wavelength: 280 nm; injection volume: 20 μL; column temperature: 29°C.
6
LC-MS Analysis of Walnut Phenolic Compounds
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LC–MS analysis was carried out as previously described [24 (link)]. LC–MS analysis was conducted in a UPLC-Triple-TOF/MS system consisting of AcquityTM ultra high performance liquid chromatograph (HPLC) (Water, Milford, CT, USA), Triple TOF 5600+ Time of Flight Mass Spectrometer (TOF) and electrospray ion source (ESI). Chromatographic separation was performed on a ZORBAX-SBC18 chromatographic column (100 mm × 4.6 mm id × 1.8 µm, Agilent, CA, USA). Mobile phases were 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). Before injection, the column was equilibrated for 5 min at initial conditions (5% B). The following condition was used: a step gradient of 5–95% B over 35 min. The injection volume and the flow rate were 5 µL and 0.8 mL/min. ESI source voltage was set at 4.5 kV in negative ionization mode maintained at 550 °C. Data was acquired from m/z 100–1500 and collected with TOF MS ~ Product Ion ~ IDA mode. Major phenolic compounds found in walnut extracts samples are shown in Table 1 and Table 2 .
7
Comprehensive Analytical Techniques for Material Characterization
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High-performance liquid chromatograph (HPLC) 1260 series with fluorescence detector (FLD) and ZORBAX SB C18 chromatographic column (150 mm × 4.6 mm; particle size: 5 μm) were manufactured by Agilent Company (Palo Alto, CA, USA). The EBI accelerator (AB5.0) was manufactured by Wuxi ELPONT Radiation Technological Co., Ltd. (Wuxi, China). X-ray diffractometer (D8) was manufactured by Bruker (China) AXS Co., Ltd., Beijing, China. High-resolution tungsten filament scanning electron microscope (SU3800) was manufactured by Techcomp (China) Scientific Instrument Co., Ltd., Tianjing, China.
8
Quantification of Methylamphetamine in Biological Samples
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The concentration of MA in serum and fecal samples from MA group mice were determined using LC–MS/MS method. In brief, 1 ml serum or fecal suspension was intensively mixed with 2 ml 0.01 mol/L Na2B4O2 and 3 ml diethyl ether. Afterward, mixtures were centrifuged (13,000 r/min, 10 min) to separate the supernatant and then, volatilized to dryness at 45°C. Samples were resolubilized with 200 μl of 70% acetonitrile solution and filtered using a 0.22 μm filter membrane. An LC-20AD Prominence UFLC system (Shimadzu, Japan) coupled with a Z-Spray electrospray interface was used to perform the chromatographic separation. Injections were made onto an Agilent Zorbax SB C18 chromatographic column (150 mm × 2.1 mm, 3.5 μm) in this study (CA, United States). The detailed procedures and parameters were set as described previously (Milesi-Hallé et al., 2015 (link); Zhang et al., 2019 (link)).
9
High-Throughput Tocopherol Quantification
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The tocopherol content in the vegetal matrix was analyzed by a new developed and validated high-throughput LC-MS/MS method. The HPLC system was an Agilent 1100 series (binary pump, autosampler, thermostat; Agilent Technologies, Santa Clara, CA, USA), coupled with a Brucker Ion Trap SL (Brucker Daltonics GmbH, Leipzig, Germany). A Zorbax SB-C18 chromatographic column (100 × 3.0 mm i.d., 3.5 μm; Agilent Technologies, Santa Clara, CA, USA) was used. The mobile phase consisted of 7:93 (v/v) water/methanol, with isocratic elution. The flow rate was 1 mL/min and the thermostat temperature set at 40 °C. The mass spectrometry detection was performed in negative mode using an atmospheric pressure chemical ionization (APCI) source and multiple-reaction monitoring (MRM) mode. A volume of 10 μL of standard solution or extract was injected into the liquid chromatography/mass spectrometry system.
10
Sensitive HPLC Analysis of Compounds
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The Agilent 1220 Infinity HPLC system (Agilent Technologies, Waldbronn, Germany) coupled with the diode-array detector and equipped with a binary pump, degasser, automatic injector, and column oven was used to perform all of the experiments. Separation was performed on the Zorbax SB C-18 chromatographic column (150 × 4.6 mm, 5 µm, Agilent Technologies, Waldbronn, Germany). The peaks corresponding to the analytes were assigned by comparing both the diode-array spectra and the retention times recorded for the real samples with the matching set of data achieved for authentic compounds. For instrument control, data acquisition, and quantitative analysis, the OpenLAB ChemStation Edition software was used. The Millipore Milli-Q-RG System (Waterford, Ireland) deionizer was used for water purification. Deionized water (Type 1) was obtained with a resistivity of 18 kΩ·cm at 25 °C. The water was filtered using a membrane filter with a pore diameter of 0.22 μm. The pH meter (Mettler-Toledo, Greifensee, Switzerland) was used to adjust the pH of the buffer solutions, for proteins removal a centrifuge with a fast cooling function (Mikro 220R, Hettich Zentrifugen, Tuttlingen, Germany) was applied, and the Labconco CentriVap (Kansas, MO, USA) was used to lyophilize the samples.
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