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N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edac

Manufactured by Merck Group
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N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC) is a chemical compound commonly used in laboratories as a coupling agent. It facilitates the formation of amide bonds between carboxyl groups and primary amines.

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Lab products found in correlation

N hydroxysuccinimide nhs, by Merck Group (4 mentions) Sulforhodamine b, by Merck Group (2 mentions) Dextran sulfate sodium salt, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Chlorhexidine dihydrochloride chx, by Merck Group (1 mentions) Pla 4032d, by NatureWorks (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Carboxygenated ames medium, by Merck Group (1 mentions) Quant it ribogreen rna assay kit, by Thermo Fisher Scientific (1 mentions) Generuler 50 bp dna ladder, by Thermo Fisher Scientific (1 mentions) Sdf 1, by Abcam (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Innoprot (1 mentions) Dna gel loading dye, by Thermo Fisher Scientific (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Raloxifene hydrochloride, by Merck Group (1 mentions) Alginic acid sodium salt from brown algae, by Merck Group (1 mentions) Low molecular weight chitosan, by Merck Group (1 mentions)

Close spelling variants of this product

N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide N-ethylcarbodiimide hydrochloride Hydrochlorides

12 protocols using n 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edac

1

Hydrogel Scaffold Fabrication Methodology

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The following materials were used to prepare the hydrogel scaffolds: gelatin (80–100 Blooms, USP-NF, BP, Ph. Eur., pure, pharmaceutical grade, PANREAC, Barcelona, Spain), HA (molecular weight of 8–10 million Dalton and purity specification of 99.94%, Kibun Food Chemifa Co., Ltd., Tokyo, Japan), sodium alginate (NF, molecular weight of 222, Spectrum®, Middleton, WI, USA), pore former of porogen sucrose (Katayama chemical Co., Osaka, Japan), cross-linkers of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC, molecular weight of 191.70 g/mole, Sigma-Aldrich, St. Louis, MO, USA), and calcium chloride (CaCl2, Shimakyu Chemical Co., Osaka, Japan). The scaffold was made using a circular mold made of acrylic cold mounting resin (Struers, Westlake, OH, USA) with a diameter of 5 cm.
Haung S.M., Lin Y.T., Liu S.M., Chen J.C, & Chen W.C. (2021). In Vitro Evaluation of a Composite Gelatin–Hyaluronic Acid–Alginate Porous Scaffold with Different Pore Distributions for Cartilage Regeneration. Gels, 7(4), 165.
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2

Synthesis of NHS-activated PDMS-b-PMOXA

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After dissolving the carbonyl terminated poly(dimethylsiloxane)-b-poly(methyloxazoline) in 5 mL of dichloromethane in a round bottom flask, the reaction mixture was cooled to 4 °C. Then, 65.4 µmol (10.2 mg) of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC, Sigma Aldrich) and 100 µmol (11.5 mg) of N-hydroxylsuccinimide (NHS, Sigma Aldrich) were added to the mixture. The final compound was recovered after performing dialysis for 24 h in DCM. The N-hydroxisuccinimide activated poly(dimethylsiloxane)-b-poly(methyloxazoline) was obtained in quantitative yield and FT-IR analysis was performed to confirm the final structure.
Ehrsam D., Porta F., Hussner J., Seibert I, & Meyer zu Schwabedissen H.E. (2019). PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism. Materials, 12(17), 2836.
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3

PLA Substrate Preparation and Functionalization

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The pellet form of poly(lactic acid) (PLA) 4032 D was obtained from Nature Works (Blair, NE) to use as a substrate. The reagents of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC), N-Hydroxysuccinimide (NHs) and Chlorhexidine dihydrochloride (CHx) were purchased from Sigma-Aldrich and their chemical compositions are given in Figure 1. PLA pellets were first dried in an oven at 60 C overnight, then pressed into the shape of sheets in 150 µm thickness by compression molding at 180 C. PLA sheets were then cut into the square form of 50 × 50 mm, gently washed with distilled water, and subsequently dried at room temperature; hereafter referred to as PLA. The solutions of 0.1% (w/v) EDAC and NHs were prepared in distilled water and CHx in 70% (v/v) isopropanol.
Ozaltin K., Di Martino A., Capakova Z., Lehocky M., Humpolicek P., Saha T., Vesela D., Mozetic M, & Saha P. (2021). Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment. Polymers, 13(8), 1201.
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4

Synthesis and Cytotoxicity Evaluation of Diverse Kinase Inhibitors

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Cumene-terminated poly(styrene-co-maleic anhydride) with an average Mn ~1,700, N-(3-dimethylaminopropyl)-N-ethyl-carbodiimide hydrochloride (EDAC), penicillin, streptomycin, and sulforhodamine B (SRB) were obtained from Sigma-Aldrich Ltd. (St Louis, MO, USA). Sorafenib (Sor), nilotinib (Nilo), crizotinib (Crizo), PD173074 (PD), pazopanib (Pazo), sunitinib (Suni), lapatinib (Lap), tofacitinib (Tofa), and selumetinib (Selu) were purchased from LC Laboratories (Woburn, MA, USA).
Archibald M., Pritchard T., Nehoff H., Rosengren R.J., Greish K, & Taurin S. (2016). A combination of sorafenib and nilotinib reduces the growth of castrate-resistant prostate cancer. International Journal of Nanomedicine, 11, 179-201.
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5

Retinal Dissection and Fixation Protocol

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Retinas from New Zealand White rabbits ranging between 3–5 kg of weight were used in this study. These animals were anesthetized with a mixture of ketamine/xylazine (40/5 mg/kg) by IM injection and euthanized with an overdose of sodium pentobarbital (100 mg/kg) following protocols approved by the Institutional Animal Care and Use Committee and conforming to National Institutes of Health (NIH) guidelines. The eyes were enucleated and the anterior segment removed along the ora serrata, followed by removal of vitreous humor. The resulting retina-sclera preparation was cut into 4 to 5 sections and the tissue pieces maintained in carboxygenated Ames’ medium (Sigma-Aldrich, St. Louis, MO) at ambient temperature. For light microscopy, tissue pieces were immersion fixed in either 4% (w/v) paraformaldehyde (PFA) or 4% N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC; Sigma-Aldrich) in 0.1M phosphate buffer (PB; pH 7.4). We found 20 minutes in 4% EDAC fixation was the best condition to visualize synaptic proteins. Retinal pieces were cryoprotected in 30% sucrose in PB overnight at 4°C, sectioned vertically at 12µm on a cryostat and collected on slides. For whole mount preparations, retinas were isolated from eyecup, flattened on a black nitrocellulose filter paper (Millipore, Billerica, MA) and fixed in 4% EDAC for 1 hour to preserve tissue integrity.
Vila A., Whitaker C.M, & O’Brien J. (2016). Membrane-Associated Guanylate Kinase scaffolds organize a horizontal cell synaptic complex restricted to invaginating contacts with photoreceptors. The Journal of comparative neurology, 525(4), 850-867.
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6

Fabrication of PLGA Nanoparticles for miR-539 Delivery

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PLGA 50:50 (503H; intrinsic viscosity 0.32–30.44 dL/g) with molecular weight of 24,000–38,000 Da was purchased from Boehringer Ingelheim International GmbH (Ingelheim am Rhein, Germany). Dichloromethane (DCM), diethyl ether, polyvinyl alcohol (PVA), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC), and N-hydroxy succinimide (NHS) were obtained from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). The miR-539 and negative control miRNA (miR-NC) were acquired from Dharmacon (GE Healthcare Dharmacon Inc. Company, Lafayette, CO, USA). iRGD (Ac-CCRGDKGPDC) with molecular weight of 1093 Da was obtained from ChinaPeptides (Shanghai, China). SDF-1 was purchased from Abcam (Cambridge, MA, USA). Furthermore, alamarBlue assay reagent was obtained from BioRad (Hercules, CA, USA). Human retinal microvascular endothelial cells (HRMECs) were obtained from the American Tissue Culture Company (Manassas, VA, USA). Endothelial cell medium kit (cat no. P60104) containing fetal bovine serum (FBS) and endothelial cell growth supplement (ECG) were obtained from Innoprot (Derio, Spain), while 0.25% trypsin with phenol red, antibiotic–antimycotic solution, and Quant-iT RiboGreen RNA Assay Kit were purchased from Invitrogen (Wilmington, DE, USA). DNA Gel Loading Dye and GeneRuler (50 bp) DNA Ladder were purchased from Thermo Scientific (Waltham, MA, USA).
Alanazi J.S., Alqahtani F.Y., Aleanizy F.S., Radwan A.A., Bari A., Alqahtani Q.H., Abdelhady H.G, & Alsarra I. (2022). MicroRNA-539-5p-Loaded PLGA Nanoparticles Grafted with iRGD as a Targeting Treatment for Choroidal Neovascularization. Pharmaceutics, 14(2), 243.
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7

Endocytosis Pathway Characterization

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Cumene terminated poly (styrene-co-maleic anhydride) with a number average molecular mass (Mn) ~1,600, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), fetal bovine serum, DiI, and biotin conjugated lectin from Ulex europaeus agglutinin (UEA-1) were obtained from Sigma-Aldrich Corp (St Louis, MO, USA). Antibodies were purchased from Cell Signaling Technology, Inc (Danvers, MA, USA). Antibodies used were: caveolin-1 (D46G3) XP® rabbit mAb #3267, clathrin heavy chain (D3C6) XP® rabbit mAb #4796, E-cadherin, and β-tubulin antibody #2146.
Parayath N.N., Nehoff H., Müller P., Taurin S, & Greish K. (2015). Styrene maleic acid micelles as a nanocarrier system for oral anticancer drug delivery – dual uptake through enterocytes and M-cells. International Journal of Nanomedicine, 10, 4653-4667.
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8

Cytokine Quantification in Murine Models

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Raloxifene hydrochloride (99% purity), cumene terminated poly(styrene-co-maleic anhydride) with an average Mn∼1600, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDAC), sulforhodamine B, dextran sulfate sodium salt (DSS were obtained from Sigma-Aldrich Ltd (Germany). Quantikine ELISA kits including Mouse IL-6 Immunoassay, Cat. No. M6000B, and Mouse TNF-α Immunoassay, Cat. No. MTA00B were purchased from R&D systems) (USA).
Greish K., Taha S., Jasim A., Elghany S.A., Sultan A., AlKhateeb A., Othman M., Jun F., Taurin S, & Bakhiet M. (2017). Styrene maleic acid encapsulated raloxifene micelles for management of inflammatory bowel disease. Clinical and Translational Medicine, 6, 28.
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9

Biomimetic Bone Graft Composite Synthesis

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The raw materials used in this study include tetracalcium phosphate (Ca4P2O9, TTCP; Realbone Technology Co., Ltd., Tainan, Taiwan), surface-modified dicalcium phosphate anhydrous (sm-DCPA; Realbone Technology Co., Ltd., Tainan, Taiwan), gelatin (80–100 Blooms (USP-NF, BP, Ph. Eur.) pure, pharma grade; PANREAC, EU), alginic acid sodium salt from brown algae (low viscosity; Sigma-Aldrich®, St. Louis, MO, USA), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC; molecular weight, 191.70 g/mole; Sigma-Aldrich®, St. Louis, MO, USA), calcium chloride (CaCl2; Shimakyu Chemical Co., Osaka, Japan), gentamicin (Siu Guan Chemical Industrial Co., Ltd., Chiayi, Taiwan), and sucrose (Katayama Chemical Co., Ltd., Osaka, Japan).
Liu S.M., Chen W.C., Ko C.L., Chang H.T., Chen Y.S., Haung S.M., Chang K.C, & Chen J.C. (2021). In Vitro Evaluation of Calcium Phosphate Bone Cement Composite Hydrogel Beads of Cross-Linked Gelatin-Alginate with Gentamicin-Impregnated Porous Scaffold. Pharmaceuticals, 14(10), 1000.
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10

Synthesis and Characterization of Bioactive Hydrogels

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Sodium CMC (0.7 carboxymethyl groups per anhydroglucose unit, MW=90,000), ethylene diamine, triethylamine (Et3N), dextran sulfate sodium salt (MW=7,000-20,000), benzydamine, fluorescein sodium salt, chitosan low molecular weight, N-Hydroxysuccinimide (NHS), and N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC) were from Sigma-Aldrich, MO, USA. Maleic anhydride, furan, and toluene were purchased from Merck & Co. (Darmstadt, Germany). Di-tert-butyl dicarbonate (Boc2O) was procured from Tokyo Chemical Industry (Tokyo, Japan). The human gingival fibroblast cell line (HGF-1) was procured from American Type Culture Collection (ATCC; Manassas, VA, USA). Dulbecco's modified Eagle's medium (DMEM), L-glutamine, non-essential amino acids, penicillin-streptomycin, and fetal bovine serum (FBS) were acquired from Gibco BRL (Rockville, MD, USA). Solvents were of analytical grade and used without purification.
Pornpitchanarong C., Rojanarata T., Opanasopit P., Ngawhirunpat T., Bradley M, & Patrojanasophon P. (2022). Maleimide-functionalized carboxymethyl cellulose: A novel mucoadhesive polymer for transmucosal drug delivery. Carbohydrate polymers, 288.
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