Analysis of differences in the expression of protein markers in fibroblasts isolated from NS and ST patients was performed using two‐sided one‐way ANOVA (IBM SPSS Statistic for Windows, version 27, IBM Corp.). The significance of differences between the kinetics of procollagen secretion, represented by the slopes of the fitted curves, was analyzed using GraphPad Prism, version 6.07 (GraphPad Software Inc.).
To evaluate differences in the collagen disc contractures, we applied repeated measures ANOVA, with a Greenhouse‐Geisser correction, to evaluate the discs' surface changes across various time points (IBM SPSS Statistic for Windows, version 27, IBM Corp.).
Our statistical analyses' results were presented in the form of graphs, tables, and descriptive statistics. In all assays, statistical significance was defined as p ≤ 0.05.