Two human head and neck cancer cell lines (FaDu and CAL-27), the human embryonic kidney cell line 293T and the mouse embryo fibroblast cell line NIH3T3 were originally obtained from ATCC (Manassas, VA, USA). The human head and neck cancer cell line SAS and the human umbilical vein endothelial cells were purchased from Bioresource Collection and Research Center of Taiwan (Hsinchu City, Taiwan). The human head and neck cancer cell line OECM-1 was kindly provided by Dr Kuo-Wei Chang (National Yang-Ming University, Taipei, Taiwan). The cell lines were not authenticated recently, and they had been confirmed without mycoplasma contamination. The expression vectors pcDNA3-myc-FBXL14 and pcDNA3-FBXL14ΔF were gifts from Professor Víctor M Díaz (Universitat Pompeu Fabra, Barcelona, Spain). pCDH-Twist1 was generated by inserting full-length complementary DNA (NM_000474.3) into pCDH-CMV-MCS-EF1-puro.
Human umbilical vein endothelial cells (huvec)
Manufactured by RIKEN BioResource Center
Sourced in United States
Human umbilical vein endothelial cells are primary cells derived from the umbilical vein of human newborns. They are a commonly used in vitro model for studying endothelial cell biology and function.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Cal27, by American Type Culture Collection (1 mentions)
Nih3t3, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Bm mscs, by PromoCell (1 mentions)
Low glucose dmem, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (1 mentions)
Gelatin, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Huvecs, by Merck Group (1 mentions)
Hyqtase, by Thermo Fisher Scientific (1 mentions)
Heparin sulfate, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
10 protocols using human umbilical vein endothelial cells (huvec)
1
Characterization of Head and Neck Cancer Cell Lines
2
Derivation and Characterization of MSCs
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PSC-MSCs were derived and characterized as previously reported [10 (link)]. ES-MSCs were derived from HSF-6 (University of California, San Francisco) and H1 (Wisconsin Alumni Research Foundation) [46 (link), 47 (link)]. iPS-MSCs were derived from iPS which were generated by two methods: (1) Sendai Virus induction (Sendai Reprogramming Kit; Invitrogen-Thermo Fisher Scientific, MA, USA) with all four Yamanaka factors into human peripheral blood mononuclear cells as per manufacturer's and published protocols [48 (link)] for iPS-MSC clones 1 and 2; and (2) lentiviral induction with Oct-4 and Sox-2 into human umbilical vein endothelial cells (Bioresource Collection and Research Center, Hsinchu, Taiwan) as previously reported [49 (link)] for iPS-MSC clone 3. BM-MSCs were obtained commercially (Promocell, Heidelberg, Germany) and cultured as previously described [4 (link)]. All MSCs were cultured with complete medium consisting of DMEM-low glucose supplemented with 1% penicillin/streptomycin, 1% L-glutamine (all from Gibco-Thermo Fisher Scientific), and 10% fetal bovine serum (FBS, selected lots from Hyclone-Thermo Fisher Scientific) and expanded as previously described [10 (link)].
3
HUVEC Isolation and Culture Protocol
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Human umbilical vein endothelial cells were purchased from Bioresource Collection and Research Center, Hsinchu, Taiwan. The culture method was modified from protocol described by Gupta et al. [30 (link)]. Briefly, HUVECs were cultured on a 1% gelatin (Sigma, MO, USA)-coated T75 flask, maintained in Dulbecco’s modified Eagle medium/Nutrient Mixture F-12 (Life Technologies, NY, USA) supplemented with 2% fetal bovine serum (Thermo, Logan, UT), 1 μg/ml hydrocortisone (Sigma, MO, USA), 5 ng/ml epidermal growth factor (PeproTech, NJ, USA), 10 ng/ml FGF-2 (PeproTech, NJ, USA), 20 μg/ml heparin sulfate (Sigma, MO, USA), 250 ng/ml insulin (Sigma, MO, USA), and 1× penicillin-streptomycin solution (Thermo, Logan, UT) at 37 °C and 5% CO2. When cells reached 80–90% confluence, they were detached using HyQtase (Thermo, Logan, UT) and replated at a ratio of 1:3.
4
Investigating Far-Infrared Radiation Effects on Cancer Cells
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Human umbilical vein endothelial cells purchased from the Bioresource Collection and Research Center were cultured in 90% medium 199 with 25 U/ml heparin and 30 μg/ml endothelial cell growth supplement (ECGS) adjusted to contain 1.5 g/L sodium bicarbonate with 10% fetal bovine serum (FBS). The prostate cancer cells (PC‐3 and DU145) and lung cancer A549 cells were purchased from American Type Culture Collection (ATCC) and cultured in RPMI‐1640 and Dulbecco's Modified Eagles Medium (DMEM) with 10% FBS, respectively. In the Cis + FIRx1 group, the cells were treated with cisplatin for 30 minutes, followed by FIR irradiation with WS TY101 FIR emitter for 30 minutes. In the Cis + FIRx2 group, the HUVECs or cancer cells were treated with cisplatin for 30 minutes, followed by exposure to FIR for 30 minutes twice with an interval of 2 hours between the two exposures. For some hypoxic tests, DU145 cells were incubated in a hypoxic chamber flushed with a gas mixture of 94% N2, 5% CO2, and 1% O2.
5
Isolation and Culture of Human Umbilical Vein Endothelial Cells
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Human umbilical vein endothelial cells were purchased from the Bioresource Collection and Research Center (Taiwan) and cultured in endothelial cell medium (ScienCell Research Laboratories).
6
Culturing Leukemia and Endothelial Cells
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The human acute myeloid leukemia cell line, HL-60, human acute lymphoblastic leukemia cell line, MOLT-4, and human umbilical vein endothelial cells (HUVEC) were obtained from Bioresource Collection and Research Center (Taiwan). Cells were maintained in RPMI-1640 medium supplemented with 10% (v/v) fetal bovine serum (Gibco, Carlsbad, CA, USA) and 1% of a mixture of penicillin-streptomycin-amphotericin B (Kibbutz Beit Haemek, Israel). For HDAC6 overexpressed cells, HL-60 cells were transfected with HDAC6-FLAG (Plasmid #13823, Addgene Inc., Cambridge, MA, USA) by using Turbofect (Thermo Fisher Scientific, Rockford, IL, USA). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2.
7
Culturing HUVEC Cells with Endothelial Medium
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8
Glioblastoma Cell Line Characterization
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The human glioblastoma cell lines LN229 and U87MG were obtained from the ATCC. LN229‐Luc2 cells were derived from a stable transfection of pLuc2‐iRFP and selected using a BD FACSAria sorter (BD Biosciences). LN229‐Luc2 cells were stably transfected with shRNA against PSMB8 and selected by puromycin. The cells were grown in RPMI‐1640 containing 10% FBS (Life Technologies) in a humidified atmosphere of 5% CO2 at 37°C. HUVEC were purchased from the Bioresource Collection and Research Center in Taiwan and cultured in endothelial cell media (ScienCell Research Laboratories).
9
Cell Culture Protocols for Cell Lines
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Human keratinocytes (HaCaT) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS. Human fibroblast WS1 cells were cultured in Eagle’s minimum essential medium containing 10% FBS. Human umbilical vein endothelial cells (HUVEC) were cultured in an endothelial cell growth medium-2 bulletkit (Lonza, Basel, Switzerland). Human dermal microvascular endothelial cells (HMEC-1) were cultured in MCDB131 medium containing 10% FBS, 10 ng/mL epidermal growth factor, 1 μg/mL hydrocortisone, and 10 mM L-glutamine. HaCaT and HMEC-1 cells were kindly provided by Professor Shinn-Jong Jiang (Department of Biochemistry, Tzu Chi University). WS1 and HUVEC were purchased from the Bioresource Collection and Research Center (Hsinchu, Taiwan). All the cell lines were incubated at 37 °C in a 5% CO2 atmosphere.
10
Cell Culture Methodology for Cancer, Fibroblast, and Endothelial Cell Lines
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The cell lines MDA-MB-468 and MCF-7 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA); HUVEC and Hs68 were from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan). The culture medium was DMEM containing 10% fetal bovine serum and 1% liquid penicillin–streptomycin. The medium was changed twice a week. The cells were kept in a 5% CO2 incubator at 37 °C.
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