C57BL/6 and C57BL/6-Tg (ACTB-EGFP) 1Osb/J mice (Jackson Laboratory) were housed at constant temperature and humidity, with a 12 : 12 h light-dark cycle and unrestricted access to a standard diet and water. When required, animals were slightly anaesthetized with sevoflurane (Abbott) or ketamine (Drag Pharma) plus xylazine (Centrovet). All procedures were in accordance with institutional and international standards for the human care and use of laboratory animals [Ethic Committee of Facultad de Medicina Clinica Alemana, Universidad del Desarrollo and Animal Welfare Assurance Publications A54427-01, Office for Protection from Research Risks, Division of Animal Welfare, NIH (National Institute of Health), Bethesda, MD, USA].
C57bl 6 tg actb egfp 1osb j mice
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
C57BL/6-Tg (ACTB-EGFP) 1Osb/J mice are a transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) under the control of the chicken beta-actin promoter and cytomegalovirus enhancer. The EGFP expression is ubiquitous in these mice, allowing for the visualization of various cell types and tissues.
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3 protocols using c57bl 6 tg actb egfp 1osb j mice
1
Murine In Vivo Anesthesia Protocol
2
Developmental stages of C57BL/6 Mice
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C57BL/6-Tg (ACTB-EGFP) 1Osb/J mice were purchased from Jackson Laboratories. Embryonic day 17.5 (E17.5), postpartum day 5 (P5), and postpartum day 28 (P28) C57BL/6 mice were obtained from HFK Bioscience Co., Ltd. (Beijing, China).
3
Isolation of Fetal Neocortex and Spinal Cord
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All the experimental protocols were approved by the Ethics Committee for Animal Research of the Koltzov Institute of Developmental Biology RAS in accordance with the Recommendations for Laboratory Practice in Russian Federation. The study was performed on C57Bl/6 and Transgenic heterozygous C57BL/6-Tg(ACTB-EGFP)1Osb/J mice (Jackson Laboratories, Bar Harbor, ME). To obtain dated pregnancy, C57Bl/6 females were caged with a male C57Bl/6-Tg(ACTB-EGFP)1Osb/J mouse. The morning on which the vaginal plug was found was designated as E0.5. Frontal neocortex tissue was obtained from E19.5 and spinal cord tissue was obtained from E14.5 fetuses harvested from transgenic EGFP mice.
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