C57bl 6 and balb c mice

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

C57BL/6 and BALB/c mice are commonly used inbred mouse strains. These mice serve as models for various research applications, including immunology, oncology, and genetic studies. Their core function is to provide consistent and genetically defined animal models for scientific investigation.

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Lab products found in correlation

C57bl 6, by Jackson ImmunoResearch (2 mentions) Balb c mice, by Jackson ImmunoResearch (1 mentions) Rag mice, by Jackson ImmunoResearch (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Sw480, by American Type Culture Collection (1 mentions) Do11.10 mice, by Jackson ImmunoResearch (1 mentions) Balb c, by Jackson ImmunoResearch (1 mentions) Global 20 protein primate diet, by Inotiv (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) One touch ultra mini glucose meter, by LifeScan (1 mentions) Matrigel, by Corning (1 mentions) Nsg mice, by Corning (1 mentions) Nsg mice, by Jackson ImmunoResearch (1 mentions) Ivis lumina 2, by PerkinElmer (1 mentions) Severe combined immunodeficiency scid mice, by Charles River Laboratories (1 mentions) Cb6f1, by Jackson ImmunoResearch (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Fluorometric hydrogen peroxide assay kit, by Merck Group (1 mentions)

Close spelling variants of this product

C57BL/6 mice (4025 citations) C57BL/6 (2462 citations) BALB/c mice (860 citations) BALB/c (473 citations) C57BL/6 and BALB/c (7 citations) Female C57BL/6 and BALB/c mice (5 citations) BALB/c × C57BL/6 (2 citations) Male Balb/c and C57BL/6 mice (2 citations) Batf3−/− BALB/c and C57Bl/6 mice (2 citations) Wild type BALB/c and C57BL/6 mice (2 citations)

47 protocols using c57bl 6 and balb c mice

Characterizing Angpt1 Knockout Mice

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Female 8-week-old C57BL/6 and BALB/c mice were obtained from the Jackson Laboratory. Angpt1 flox (floxed), Angpt1 del, ROSA-rtTA (ROSA), and tet-O Cre (Cre) mice (17 (link)) were backcrossed with BALB/c mice from the Jackson Laboratory for 10 generations. Mice hemizygous for the ROSA-rtTA and tetO-Cre transgene but not the Ang-1^flox or del allele were used as wild-type controls (Angpt1^+/+).
Transgene mice were treated with doxycycline via drinking water ad libitum for 14 days to induce floxed exon gene excision, as assessed by Ang-1 ELISA (R&D Systems). Mice were kept on normal drinking water for 2 weeks before initiation of PbA experiments to clear doxycycline, which has antimalarial activity. Both male and female Angpt1^−/− mice were used for experimental analysis (with littermate, sex, and age-matched Angpt1^+/+ controls).

Higgins S.J., Purcell L.A., Silver K.L., Tran V., Crowley V., Hawkes M., Conroy A.L., Opoka R.O., Hay J.G., Quaggin S.E., Thurston G., Liles W.C, & Kain K.C. (2016). Dysregulation of angiopoietin-1 plays a mechanistic role in the pathogenesis of cerebral malaria. Science translational medicine, 8(358), 358ra128.

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Murine Models for Immunology Research

Cited 1 time

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C57BL/6 and Balb/c mice (6–8-weeks old), Rag^−/− mice and CCR2^−/− on C57BL/6 background were purchased from Jackson Laboratory. Constitutive AHR knockout mice were obtained from Taconic. Pmel-1 TCR transgenic mice have been reported^{45 (link),46 (link)} and were provided by N. Restifo (National Cancer Institute (NIH), Bethesda, MD). Foxp3^GFP knock-in mice were a gift from Dr. A. Rudensky (Memorial Sloan Kettering Cancer Center (MSKCC), New York, NY). Foxp3^DTR mice were generated in the laboratory of Dr. G. J. Hammerling (German Cancer Research Center (DKFZ), Heidelberg, Germany), and previously described^{47 (link)}. All mice were maintained in microisolator cages and treated in accordance with the NIH and American Association of Laboratory Animal Care regulations. All mouse procedures and experiments for this study were approved by the MSKCC Institutional Animal Care and Use Committee.

Campesato L.F., Budhu S., Tchaicha J., Weng C.H., Gigoux M., Cohen I.J., Redmond D., Mangarin L., Pourpe S., Liu C., Zappasodi R., Zamarin D., Cavanaugh J., Castro A.C., Manfredi M.G., McGovern K., Merghoub T, & Wolchok J.D. (2020). Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine. Nature Communications, 11, 4011.

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Murine Acclimation and Housing

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Pathogen-free, male C57BL/6 and Balb/c mice (Jackson Laboratory) weighing 20–24 g were used. All animals were housed in the specific pathogen-free facility and had access to water and food ad libitum. The mice were housed in cages under hygienic conditions and kept at room temperature (19–25 °C) with a 12 h light/12 h dark cycle. Mice were acclimated for 1 week before the experiment.

Pandolfi L., Fusco R., Frangipane V., D’Amico R., Giustra M., Bozzini S., Morosini M., D’Amato M., Cova E., Ferrario G., Morbini P., Colombo M., Prosperi D., Viglio S., Piloni D., Di Paola R., Cuzzocrea S, & Meloni F. (2020). Loading Imatinib inside targeted nanoparticles to prevent Bronchiolitis Obliterans Syndrome. Scientific Reports, 10, 20726.

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Cell Culture Protocols for Colorectal Cancer

Cited 1 time

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HT29, HCT116, SW480 and CT26 were purchased from ATCC. HT29 and HCT116 cells were maintained in McCoy’s 5A medium containing 2 mmol/L L-glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin, and 10% FBS. SW480 and CT26.WT media were cultured in RPMI-1640 medium containing 2 mmol/L L-glutamine, 100 U/mL penicillin, 100 mg/mL streptomycin, and 10% FBS. MC38 cells were kindly provided by Dr. Shari Pilon-Thomas (Moffitt Cancer Center) and were cultured in complete medium as recommended^{35 (link)}. KM12 cells were obtained from Dr. Bhaumik Patel, Richmond, VA. All cells were maintained in an atmosphere containing 5% CO₂ and at 37 °C. In addition, cells were routinely checked for mycoplasma contamination. C57BL/6 and BALB/c mice were purchased from The Jackson Laboratory.

Quarni W., Dutta R., Green R., Katiri S., Patel B., Mohapatra S.S, & Mohapatra S. (2019). Mithramycin A Inhibits Colorectal Cancer Growth by Targeting Cancer Stem Cells. Scientific Reports, 9, 15202.

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AOM/DSS-Induced Colorectal Cancer in Mice

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Three to four-week-old female C57BL/6 and BALB/c mice purchased from Jackson Laboratory were used in this study. On arrival at the animal facility, mice were randomly assigned to restored or unrestored groups (n = 5/group) and received an ear punch. Bedding on days 1–3 was pooled between experimental groups to normalize the baseline microbiome between restored and unrestored groups. Following baseline normalization, mice underwent AOM/DSS treatment, and stool samples were longitudinally collected 3x/week for 18 weeks. In week 18, mice were sacrificed and dissected. Mouse colons were fixed in 10% neutral buffered formalin and processed for histological analysis. All mice were housed in specific pathogen-free conditions in fully autoclaved cages for the duration of the experiment. The Institutional Animal Care and Use Committee approved all animal studies.

Gates T.J., Yuan C., Shetty M., Kaiser T., Nelson A.C., Chauhan A., Starr T.K., Staley C, & Subramanian S. (2023). Fecal Microbiota Restoration Modulates the Microbiome in Inflammation-Driven Colorectal Cancer. Cancers, 15(8), 2260.

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Generation and Characterization of Genetically Modified Mice

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Mice were bred and maintained in the specific pathogen-free (SPF) animal facility at UT MD Anderson Cancer Center in accordance with institutional guidelines. We generated RORγ^f/f mice, in which exon 2 and 3 of Rorc gene are flanked by LoxP sites. The resultant RORγ^f/f mice were bred to FLPeR mice to remove Neomycin resistance cassette and then back-crossed to C57BL/6 mice for six generations. Foxp3^YFP-Cre knock-in mice and Foxp3^GFP reporter mice were kindly provided by Dr. Alexander Rudensky (Memorial Sloan-Kettering Cancer Center). Foxp3^GFP-Cre BAC transgenic mice were kindly provided by Drs. Jeffrey Bluestone (UCSF) and Shao-Cong Sun (UT MD Anderson Cancer Center). Stat3^f/fCD4^Cre and B7h^−/− mice were previously described (Nurieva et al., 2003 (link); Yang et al., 2007 (link)). C57BL/6 and BALB/c mice were purchased from Jackson Laboratories. Foxp3^GFP reporter mice on BALB/c background and DO11.10 mice were obtained from Jackson Laboratories and were crossed in our animal facility. Female and male mice at 8–12 weeks of age were used for experiments.

Kim B.S., Lu H., Ichiyama K., Chen X., Zhang Y.B., Mistry N.A., Tanaka K., Lee Y.H., Nurieva R., Zhang L., Yang X., Chung Y., Jin W., Chang S.H, & Dong C. (2017). Generation of RORγt+ antigen-specific T regulatory 17 (Tr17) cells from Foxp3+ precursors in autoimmunity. Cell reports, 21(1), 195-207.

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Pathogen-Free Murine Experiments

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C57BL/6 and Balb/c mice were obtained from the Jackson Laboratory (Bar Harbor, ME, United States). All mice were kept under specific pathogen-free conditions. All experiments had the approval of the Institutional Animal Care and Use Committee at Michigan State University. Both male and female mice (6–8 weeks old) were used for experiments.

Callahan B.N., Kammala A.K., Syed M., Yang C., Occhiuto C.J., Nellutla R., Chumanevich A.P., Oskeritzian C.A., Das R, & Subramanian H. (2020). Osthole, a Natural Plant Derivative Inhibits MRGPRX2 Induced Mast Cell Responses. Frontiers in Immunology, 11, 703.

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Transgenic Mouse Models for Tumor Studies

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6- to 8-week-old female mice were used at the outsets of all experiments. Pmel (B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J) and OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J) transgenic mice were bred in house using breeding pairs purchased from Jackson Lab. C57BL/6 and BALB/c mice for tumour studies were purchased from Jackson Lab. All animal procedures were approved by Georgia Tech IACUC (protocol #KWONG-A100193).

Mac Q.D., Sivakumar A., Phuengkham H., Xu C., Bowen J.R., Su F.Y., Stentz S.Z., Sim H., Harris A.M., Li T.T., Qiu P, & Kwong G.A. (2022). Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors. Nature biomedical engineering, 6(3), 310-324.

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Generating Muc5ac Knockout Mouse Lines

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Studies were performed with approval of the animal care and use committees of the University of Colorado (approval number 97412(01)E) and the MD Anderson Cancer Center (approval numbers 05-04-046, 05-05-04334, and 00001214-RN00). Muc5ac^−/− mice were generated previously^{37 (link)}. Mice were backcrossed onto congenic C57BL/6 and BALB/c lines using marker assisted analysis at the MD Anderson Research Animal Support Facility-Smithville, Animal Genetics Services lab. After achieving 100% carriage of 77 C57BL/6 and 82 BALB/c strain specific markers, lines were backcrossed 3-5 additional generations. Mice were either bred as homozygous nulls or heterozygotes. C57BL/6 and BALB/c mice from the Jackson Labs (Bar Harbor, ME) were used for congenic backcrossing and as WT controls. Male and female mice were used starting at 7-8 wks age. Before and after exposures, mice were housed under specific pathogen-free conditions in ventilated cages (≤5 mice per cage) with a 12 h light/dark cycle and with food and water available ad libitum. Mice were placed into experimental and control groups chronologically.

Evans C.M., Raclawska D.S., Ttofali F., Liptzin D.R., Fletcher A.A., Harper D.N., McGing M.A., McElwee M.M., Williams O.W., Sanchez E., Roy M.G., Kindrachuk K.N., Wynn T.A., Eltzschig H.K., Blackburn M.R., Tuvim M.J., Janssen W.J., Schwartz D.A, & Dickey B.F. (2015). The polymeric mucin Muc5ac is required for allergic airway hyperreactivity. Nature communications, 6, 6281.

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Comparative Murine Thymus Extraction

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C57BL/6 and Balb/c mice (The Jackson Laboratory, Bar Harbor, ME) were kept under pathogen free conditions, sacrificed at three weeks of age and thymus tissue was retrieved. The work was surveyed and approved by the Gothenburg University ethics committee (ethics approval no: 2012–158). The methods were carried out in accordance with the approved guidelines.

Lundberg V., Berglund M., Skogberg G., Lindgren S., Lundqvist C., Gudmundsdottir J., Thörn K., Telemo E, & Ekwall O. (2016). Thymic exosomes promote the final maturation of thymocytes. Scientific Reports, 6, 36479.

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