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Erk1 sc 271270

Manufactured by Santa Cruz Biotechnology
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ERK1 (sc-271270) is a lab equipment product manufactured by Santa Cruz Biotechnology. It is a protein-specific antibody that recognizes the ERK1 (extracellular signal-regulated kinase 1) protein.

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Lab products found in correlation

C myc sc 40, by Santa Cruz Biotechnology (2 mentions) Zeb1 nbp 1 05987, by Novus Biologicals (2 mentions) Nanog, by Cell Signaling Technology (2 mentions) Phospho erk1 2, by Cell Signaling Technology (2 mentions) Snail, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Ripa buffer, by Merck Group (2 mentions) E cadherin, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) E cadherin, by BD (2 mentions) Mek1 2 ser217 221, by Cell Signaling Technology (1 mentions) Phospho mek1 2 ser217 221, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Protein assay, by Bio-Rad (1 mentions)

2 protocols using erk1 sc 271270

1

Western Blot Analysis of Stemness and EMT Markers

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Samples were collected in RIPA buffer (Sigma) containing Complete Protease Inhibitor Cocktail (Roche Diagnostics), and protein concentration was determined by Bio-Rad Protein Assay. Western blot analysis was performed using the following antibodies: KRAS (sc-30), Pan-RAS (sc-32), ERK1 (sc-271270), and c-Myc (sc-40) from Santa Cruz Biotechnology; Sox2 (#2748, #3579), Oct-4 (#2750), Nanog (#4893), Slug (#9585), Snail (#3879), phospho-ERK1/2 (#9101), RAS (#3965), MEK1/2 (Ser217/221) (#4694), phospho-MEK1/2 (Ser217/221) (#9121, #9154), CD44 (#3578, #3570), E-cadherin (#14472), vimentin (#3932) and cleaved caspase-3 (#9661) from Cell Signaling; N-cadherin (BD610920) and E-cadherin (BD610181) from BD Biosciences; Zeb1 (NBP-1-05987) from Novus Biologicals; and β-actin from Sigma.
Yoon C., Till J., Cho S.J., Chang K.K., Lin J.X., Huang C.M., Ryeom S, & Yoon S.S. (2019). KRAS activation in gastric adenocarcinoma stimulates epithelial-to-mesenchymal transition to cancer stem-like cells and promotes metastasis. Molecular cancer research : MCR, 17(9), 1945-1957.
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2

Western Blot Analysis of Cellular Markers

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Samples were collected in radioimmunoprecipitation (RIPA) buffer (Sigma-Aldrich) containing Complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland), after which protein concentrations were determined by the Bio-Rad Protein Assay (Bio-Rad Laboratories, Hercules, CA). Western blotting was performed using the following antibodies, ERK1 (sc-271270), ERK2 (sc-136288), and c-Myc (sc-40) from Santa Cruz Biotechnology; Sox2 (#2748, #3579), Oct-4 (#2750), Nanog (#4893), Slug (#9585), Snail (#3879), phospho-ERK1/2 (#9101), CD44 (#3578, #3570), E-cadherin (#14472), ERK1/2 (#4696), and cleaved caspase-3 (#9661) from Cell Signaling Technology (Danvers, MA); N-cadherin (BD610920) and E-cadherin (BD610181) from BD Biosciences; Zeb1 (NBP-1-05987) from Novus Biologicals (Centennial, CO); and β-actin from Sigma-Aldrich.
Huang C., Yoon C., Zhou X.H., Zhou Y.C., Zhou W.W., Liu H., Yang X., Lu J., Lee S.Y, & Huang K. (2020). ERK1/2-Nanog signaling pathway enhances CD44(+) cancer stem-like cell phenotypes and epithelial-to-mesenchymal transition in head and neck squamous cell carcinomas. Cell Death & Disease, 11(4), 266.
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