Goat anti ccr5 antibody

Maraviroc was obtained from Sigma-Aldrich (St. Louis, MO, USA) (for in vitro experiments) or GlaxoSmithKline (Brentford, UK) (for in vivo experiments). Mouse CCL3 was obtained from Peprotech (Rocky Hill, NJ, USA). The following antibodies were used as the primary antibodies for immunohistochemical analyses: goat anti-CCR5 antibody (Santa Cruz Biotechnology, Dallas, TX, USA), rat anti-Ly6G antibody (BD Biosciences, San Jose, CA, USA), rat anti-F4/80 antibody (Serotec, Kidlington, UK), mouse anti-α-SMA antibody (Dako, Glostrup, Denmark), rabbit anti-type I collagen antibody, rabbit anti-CD31 antibody and rabbit anti-EGF antibody (Abcam, Cambridge, UK). The following rat anti-mouse antibodies were used as the primary antibodies for the flow cytometric analysis: anti-CD11b antibody (BD Biosciences), anti-CD25 antibody (BioLegend, San Diego, CA, USA), anti-CD45 antibody (eBioscience, San Diego, CA, USA), anti-F4/80 antibody (eBioscience), anti-Foxp3 antibody (eBioscience), anti-Ly6G antibody (Gr-1) (Tonbo Biosciences, San Diego, CA, USA), anti-MIP-1α antibody (R&D Systems, Minneapolis, MN, USA), anti-CCR5 antibody (BioLegend), and isotype-matched control IgGs for individual rat antibodies (BD Biosciences).

The avidin-biotin technique was applied as previously described (31 (link)). After H₂O₂ treatment, paraffin-embedded sections were blocked for 30 min with 10% donkey serum at room temperature and incubated with the related anti-RANTES primary antibodies (5 mg/mL) overnight at 4°C, then followed by the 1:300-diluted biotinylated secondary antibody. The negative controls were performed in the absence of primary antibody. The same method was used in the experiment to detect the localization of CCR1 and CCR5 in rat epididymis, where the goat anti-CCR1 antibody and goat anti-CCR5 antibody (Santa Cruz Biotechnology) were used, with secondary antibodies, biotinylated anti-goat IgG (Sigma, St. Louis, MO, United States), applied for immunohistochemical staining.