Autodelfia insulin kit

After a 12-h overnight fast, participants in study groups 1 and 4 underwent a standard 75 g OGTT. Serum insulin and plasma glucose was measured at 0, 30 and 120 minutes. Serum insulin levels (excluding des-31,32 and intact proinsulin) were measured using the AutoDELFIA insulin kit (Perkin-Elmer, Wallac, Turku, Finland). Plasma glucose was analyzed using a glucose oxidase method (Granutest; Merck, Darmstadt, Germany) [26 (link)].

The blood samples were drawn after a 12h fast for measures of glycated hemoglobin (HbA1c), plasma glucose, serum insulin, serum C-peptide, total cholesterol and serum triglyceride [16 (link), 20 (link)]. In addition, individuals without known diabetes underwent a standard 75g OGTT with samples drawn at 30, and 120 minutes measured for plasma glucose, serum insulin and C-peptide. Serum insulin levels (excluding des-31,32 and intact proinsulin) were measured using the AutoDELFIA insulin kit (Perkin-Elmer, Wallac, Turku, Finland). Plasma glucose was analysed using a glucose oxidase method (Granutest; Merck, Darmstadt, Germany)[17 (link)]. Concentrations of serum triglycerides and total cholesterol were analysed using enzymatic colorimetric methods (GPO-PAP and CHOD-PAP, Roche Molecular Biochemicals, Germany). HbA1c was measured using ion-exchange high performance liquid chromatography (normal reference range: 4.1–6.4%) [21 (link)].