Preservcyt

Manufactured by Qiagen

Sourced in United States, Germany

PreservCyt is a liquid-based cytology preservative solution used for the collection, transportation, and storage of cellular samples. Its primary function is to maintain the integrity and morphology of cells collected for cytological examination.

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Lab products found in correlation

Preservcyt, by Hologic (2 mentions) Puregene buccal cell kit, by Qiagen (1 mentions) Thinprep t2000, by Hologic (1 mentions)

3 protocols using preservcyt

Optimizing Cervical Cytology Sample Preparation

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HPV-positive HeLa and HPV-negative C33A cells were reconstituted in two sample collection buffers commonly used for cervical cytology specimens, namely SurePath preservation buffer (Becton Dickinson, Franklin Lakes, NJ) and PreservCyt (Hologic, Marlborough, MA). Prior to sample preparation, preserved cells were converted to a Tris-based buffer. For samples preserved in SurePath, 1 mL of sample was centrifuged at 4000 RPM for 10 minutes, samples were resuspended in 10 mM Tris, and heated at 120 °C for 20 minutes to reverse formalin-induced crosslinking. For samples preserved in PreservCyt, samples were converted to 10 mM Tris using the Sample Conversion Kit (Qiagen, Germantown, MD) per kit instructions. After conversion, positive (HeLa) and negative (C33A) controls were tested on the HPV DNA paper assay to determine buffer compatibility (Fig. S2†).

Smith C.A., Chang M.M., Kundrod K.A., Novak E.N., Parra S.G., López L., Mavume C., Lorenzoni C., Maza M., Salcedo M.P., Carns J.L., Baker E., Montealegre J., Scheurer M., Castle P.E., Schmeler K.M, & Richards-Kortum R.R. (2022). A low-cost, paper-based hybrid capture assay to detect high-risk HPV DNA for cervical cancer screening in low-resource settings. Lab on a Chip, 23(3), 451-465.

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Cell Preservation and DNA Extraction

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After withdrawal, the sponge was placed in a vial containing 20 mL of a cell preservative buffer (PreservCyt, Hologic, Marlborough, Mass, USA) and agitated to dislodge cells once received in the laboratory, as previously described.^{13 (link)} This step was repeated with an additional 20 mL aliquot of PreservCyt for a total of 40 mL. The cells were pelleted by centrifugation of the sample (at 1200 G force for 10 minutes), the pellet then lysed in 1 mL of lysis buffer (Puregene Buccal Cell Kit, Qiagen, Germantown Md, USA).

Iyer P.G., Taylor W.R., Slettedahl S.W., Lansing R.L., Hemminger L.L., Cayer F.K., Mahoney D.W., Giakoumopoulos M., Allawi H.T., Wu T.T., Wang K.K., Wolfsen H.C., Antpack E, & Kisiel J.B. (2021). Validation of a methylated DNA marker panel for the nonendoscopic detection of Barrett’s esophagus in a multi-site case-control study. Gastrointestinal endoscopy, 94(3), 498-505.

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Cervical Cancer Screening Protocol

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Thin-layer cytology slides were prepared using the Thinprep T2000 slide processor (Hologic) and stained using the Papanicolaou method. Cytology slides were evaluated by a cytotechnologist and confirmed by a pathologist using the revised Bethesda nomenclature. (26) Formalin-fixed, paraffinembedded 4-mm sections were routinely stained with hematoxylin and eosin (H&E). All the histological samples were reviewed by one of the authors (JO) to confirm the presence or absence of cervical lesion and its grade. The histological diagnoses were established using pure morphologic criteria based on the H&E-stained sections, with no knowledge of HPV status or the cytology result. The LAST nomenclature was used for the histological diagnosis. (27) Routine HPV detection (hybrid capture II) Detection of hr-HPV was performed in cytological samples. Initially hr-HPV detection was undertaken with the Hybrid Capture 2 (HC2) system (Qiagen, Hilden, Germany) in the samples collected in liquid-based media (PreservCyt). This test detects the following genotypes: 16, 18, 32, 34, 36, 39, 45, 51, 52, 56, 58, 59 , and 68. A relative light unit of 1 (1.0 pg/mL) was used as the cut-off to classify a specimen as positive for hr-HPV. (28)

Del Pino M., Alonso I., Rodriguez-Trujillo A., Bernal S., Geraets D., Guimerà N., Torne A, & Ordi J. (2017). Comparison of the analytical and clinical performance of five tests for the detection of human papillomavirus genital infection. Journal of virological methods, 248.

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