Erastin2

SNU16 and 293T cells were purchased from ATCC. SNU668 cells were obtained from the Korean Cell Line Bank (KCLB). SNU16 and SNU668 cells were cultured in RPMI containing 10% fetal bovine serum (FBS), 100 units ml⁻¹ penicillin, and 100 μg ml⁻¹ streptomycin (Pen/Strep). 293T cells were cultured in DMEM containing 10% FBS, Pen/Strep, and 2 mM L-glutamine. Cells were kept in an incubator maintained at 37°C and 5% CO₂. Cystine starvation media was prepared as previously described[18 (link)]. Erastin2 (Cayman Chemical Company), RSL3, and Lpx-1 (Selleck Chemicals) were all dissolved in DMSO. For experiments, “sparse” is defined as 5 × 10³ (SNU16) or 3 × 10³ (SNU668) cells in a 96-well and 8 × 10⁴ (SNU16) or 5 × 10⁵ (SNU668) in a 6-well dish. “Dense” is defined as 5 × 10⁴ (SNU16) or 3 × 10⁴ (SNU668) cells in a 96-well and 8 × 10⁵ (SNU16) or 5 × 10⁵ (SNU668) in a 6-well dish. Cells were grown for 30 hours prior to use for experiments. All cell lines used were sent for STR profiling and routinely monitored for mycoplasma contamination.