Aoc 20i s type autosampler

The qualification of the sample extract was performed using a GC-MS/MS system (GCMS-TQ8040; Shimadzu, Kyoto, Japan) equipped with a ZB5-MSi fused-silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Phenomenex, Torrance, CA, USA). Grade 5.0 helium was used as the carrier gas. Column flow was 1 mL/min. The injection of a 1 μL sample was performed using an AOC-20i + s type autosampler (Shimadzu, Kyoto, Japan). The injector was working at a temperature of 310 °C. The following temperature program was applied: the oven temperature was held at 60 °C for 2 min and was subsequently increased linearly at a rate of 6 °C/min to 310 °C, where it was held for 15 min. The mass spectrometer was operated in EI mode at 70 eV; the ion source temperature was 225 °C. The mass spectra were measured in the range of 40–450 amu. The amounts of the individual analytes were estimated with the peak normalization method.

The qualification of the sample extract was performed using a GC-MS/MS system (GCMS-TQ8040; Shimadzu, Kyoto, Japan) equipped with a ZB5-MSi fused-silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Phenomenex, Torrance, CA, USA). Grade 5.0 helium was used as the carrier gas. Column flow was 1 mL/min. The injection of a 1 μL sample was performed using an AOC-20i + s type autosampler (Shimadzu, Kyoto, Japan). The injector was working at a temperature of 310 °C. The following temperature program was applied: the oven temperature was held at 60 °C for 2 min and was subsequently increased linearly at a rate of 6 °C/min to 310 °C, where it was held for 15 min. The mass spectrometer was operated in EI mode at 70 eV; the ion source temperature was 225 °C. The mass spectra were measured in the range 40–450 amu. The amounts of the individual analytes were estimated by the peak normalization method.