Anti vdac1 4661

rhIGFBP-3 was acquired from Sino Biological (Chesterbrook, PA, USA). The lyophilized protein was re-suspended using ultrapure water. Antibodies used for immunoblotting and immunofluorescence included: a rabbit polyclonal anti-IGF-1Rβ #3027, a rabbit monoclonal anti-mitofusion-2 #9482, a rabbit monoclonal anti-mitofusion-1 #14739, a rabbit monoclonal anti-OPA1 #67589, a rabbit monoclonal anti-COX IV #4850, a rabbit polyclonal anti-VDAC1 #4661 (Cell Signaling, Danvers, MA, USA); a mouse monoclonal anti-GAPDH #sc-66163, a mouse monoclonal anti-β-actin #sc-47778 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and a mouse monoclonal anti-puromycin #MABE343 (EMB Millipore, Burlington, MA, USA). Secondary antibodies used for immunofluorescence were anti-rabbit IgG conjugated to Alexa Fluor 488 and anti-mouse IgG conjugated to Alexa Fluor 555 (Cell Signaling, Danvers, MA, USA). For immunoblotting, secondary antibodies were goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP) #170-6515 and goat anti-mouse conjugated to HRP #170-6516 (Bio-rad, Hercules, CA, USA).