Ff495

Manufactured by IDEX Corporation

The FF495 is a high-quality laboratory equipment product manufactured by IDEX Corporation. It is designed to perform core functionalities for laboratory applications. Detailed technical specifications and intended use are not available without further information.

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Lab products found in correlation

Fiber launch, by Thorlabs (1 mentions) Sr810 dsp, by Stanford Research Systems (1 mentions) Ni pcie 6321, by National Instruments (1 mentions) Mfc 400 430, by Doric (1 mentions) Sr810, by Stanford Research Systems (1 mentions)

Spelling variants of this product

FF495-Di03 (6 citations) FF495-Di02−25 × 36 (5 citations) FF495-Di03-25 × 36 (4 citations) DC FF495-DI02 (3 citations) FF495-Di03-50.8-D dichroic mirror (3 citations) FF495-Di02-25 3 36 (2 citations) FF495-Di03-25x36 (2 citations)

3 protocols using ff495

Fiber Photometry Recording of GCaMP Signals

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The fiber photometry system used a 473 nm diode laser (Omicron Luxx) that was chopped at 400 Hz, reflected off a dichroic (Semrock, FF495), and coupled into a 400 um 0.48 NA optical fiber using a 40× 0.65 NA microscope objective (Olympus) and fiber launch (Thorlabs). The laser intensity at the interface between the fiber tip and the animal ranged from 1.8–1.9 mW (but was constant across trials that were compared side by side). GCaMP fluorescence collected by the objective and transmitted by the dichroic was focused through a bandpass filter (Semrock, FF01–520/35) onto a NewFocus 2151 femtowatt silicon photoreceiver (Newport, DC Low mode), the output of which was directed through a lock-in amplifier (SR810 DSP, Stanford Research Systems, 3 ms time constant), digitized using a LabJack DAQ, and recorded by custom Python software. Signals were collected at a sampling frequency of 250 Hz.

Gunaydin L.A., Grosenick L., Finkelstein J.C., Kauvar I.V., Fenno L.E., Adhikari A., Lammel S., Mirzabekov J.J., Airan R.D., Zalocusky K.A., Tye K.M., Anikeeva P., Malenka R.C, & Deisseroth K. (2014). Natural neural projection dynamics underlying social behavior. Cell, 157(7), 1535-1551.

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Fibre Photometry for Calcium Imaging

Cited 2 times

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Fibre photometry uses the same optic fibre to both excite and record from the genetically encoded calcium indicator GCaMP6f in real time and thus can allow for temporally precise measurements of neuronal activity that can then be time-locked to a specific behavioural output26 (link)27 (link)42 (link). Our fibre photometry system used two LEDs at 490 and 405 (Thor Labs), reflected off dichroic mirrors (Semrock, FF495) and coupled into a 400 μm 0.48 NA optical fibre (Thorlabs BFH48–600, important for minimal autofluorescence and signal recovery) using a 40 × 0.48 NA microscope objective (Olympus) and fibre launch (Thorlabs), with the patchcord linked to an implanted 400 μm optical fibre with zirconia sheath. The LED intensity at the interface between the fibre tip and the animal ranged from 30 to 75 μW (but was constant across trials and over days). The power output of the system was tested with a power meter at the start of each experimental session. A real-time signal processor (RX8, Tucker-Davis Technologies, Alachua FL), running software that was custom designed using OpenEx, sinusoidally modulated each laser's output. The two output signals were projected onto a photodetector (Model 2151 Femtowatt Photoreceiver, Newport, Irvine, CA) after which they were separated for analysis. Signals were collected at a sampling frequency of 381 Hz.

Calipari E.S., Juarez B., Morel C., Walker D.M., Cahill M.E., Ribeiro E., Roman-Ortiz C., Ramakrishnan C., Deisseroth K., Han M.H, & Nestler E.J. (2017). Dopaminergic dynamics underlying sex-specific cocaine reward. Nature Communications, 8, 13877.

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Fiber photometry setup for calcium imaging

Cited 1 time

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The fiber photometry setup was similar to that described in Gunaydin et al. (2014) (link). Briefly, a fiber-coupled 470 nm LED (Thorlabs, M470F3) was reflected off of a dichroic mirror (Semrock, FF495) into a 600 μm, 0.48 NA optical fiber patch-cord (Doric). The patch-cord was then coupled to a 400 μm, 0.48 NA fiber (Doric, MFC_400/430-0.48_MF2.5_FLT) implanted in the mouse’s brain. The resulting GCaMP or GFP fluorescence was transmitted through the dichroic mirror and band-pass filter (Semrock, FF01-F20/35), and focused via a convex lens (Thorlabs, LA1255A) onto a photo-detector (Newport, 2151). A lock-in amplifier (Stanford Research Systems, SR810) was used to modulate the LED at 400 Hz, and then demodulate the resulting signal collected from the photo-detector. The demodulated signal was digitized at 1 kHz using a data acquisition board (National Instruments, NI PCIe-6321).

Lovett-Barron M., Andalman A.S., Allen W.E., Vesuna S., Kauvar I., Burns V.M, & Deisseroth K. (2017). Ancestral Circuits for the Coordinated Modulation of Brain State. Cell, 171(6), 1411-1423.e17.

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