Immunocap phadiatop infant

Manufactured by Thermo Fisher Scientific

Sourced in Sweden

The ImmunoCAP Phadiatop Infant is a laboratory diagnostic tool designed to detect the presence of specific IgE antibodies in infant blood samples. It is used to screen for a range of common environmental and food allergens.

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Lab products found in correlation

Immunocap, by Thermo Fisher Scientific (13 mentions) Immunoglobulin e elisa kit, by Immundiagnostik (2 mentions) Centrifuge 5810r, by Eppendorf (1 mentions)

18 protocols using immunocap phadiatop infant

Measurement of Total and Allergen-Specific IgE

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Total serum immunoglobulin E (IgE) level was measured by ImmunoCAP (Phadia, Uppsala, Sweden), while allergen-specific IgE was determined using a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia). The most common food allergens (egg white, milk, and wheat) and inhalant allergens (Dermatophagoides pteronyssinus, Dermatophagoides farina, and Cladosporium herbarum) were selected and measured.^{19 (link)} Allergic sensitization was defined as ImmunoCAP Phadiatop Infant values ≥0.35 kU/L.^{20 (link)}

Chiu C.Y., Liao S.L., Su K.W., Tsai M.H., Hua M.C., Lai S.H., Chen L.C., Yao T.C., Yeh K.W, & Huang J.L. (2016). Exclusive or Partial Breastfeeding for 6 Months Is Associated With Reduced Milk Sensitization and Risk of Eczema in Early Childhood: The PATCH Birth Cohort Study. Medicine, 95(15), e3391.

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Serum IgE Measurement for Allergy Diagnosis

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Total and allergen-specific serum IgE were examined as described in our previous study [18 (link)]. Total serum IgE level was measured by ImmunoCAP (Phadia, Uppsala, Sweden) and IgE sensitization was defined as IgE levels ≥ 100 kU/L [23 ]. Three food allergens (egg white, milk and wheat) and three inhalant allergens (D. pteronyssinus, D. farina and C. herbarum) were measured simultaneously [24 (link)]. Allergen-specific IgE was determined using a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia) and allergen sensitization was defined as values ≥ 0.35 kU/L [25 (link)].

Yeh K.W., Chiu C.Y., Su K.W., Tsai M.H., Hua M.C., Liao S.L., Lai S.H., Chen L.C., Yao T.C, & Huang J.L. (2016). High cord blood CCL22/CXCL10 chemokine ratios precede allergic sensitization in early childhood. Oncotarget, 8(5), 7384-7390.

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Infant Allergen Sensitization Assessment

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Serum level of total immunoglobulin E and allergen-specific IgE were measured by ImmunoCAP (Phadia, Uppsala, Sweden) and a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia) respectively at 6 months, and 1 and 2 years of age. Two most common food allergens, cow’s milk, and egg white were measured. Allergic sensitization was defined as ImmunoCAP Phadiatop Infant values ≥ 0.35 kU/L [30 (link)].

Tang C.M., Lin G., Chiang M.H., Yeh K.W., Huang J.L., Su K.W., Tsai M.H., Hua M.C., Liao S.L., Lai S.H, & Chiu C.Y. (2022). Longitudinal Metabolomic Analysis Reveals Gut Microbial-Derived Metabolites Related to Formula Feeding and Milk Sensitization Development in Infancy. Metabolites, 12(2), 127.

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Allergen-specific IgE Determination

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Allergen-specific IgE was determined using a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia) for a mix of the two most common food allergens (egg white and milk) and inhalant allergens in Taiwan (D. pteronyssinus and D. farinae) as described previously^{22 (link),23 (link)}. Allergen sensitization was defined as values ≥ 0.35 kU/L^{24 (link)}.

Wong C.Y., Yeh K.W., Huang J.L., Su K.W., Tsai M.H., Hua M.C., Liao S.L., Lai S.H., Chen L.C, & Chiu C.Y. (2020). Longitudinal analysis of total serum IgE levels with allergen sensitization and atopic diseases in early childhood. Scientific Reports, 10, 21278.

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Allergic Sensitization in Infants

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Total IgE level was determined by ImmunoCAP (Phadia, Uppsala, Sweden) at birth and by age of 1 year. Specific IgE was determined by a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia) against the most common inhalant and food allergens (i.e. house dust mite, cat, dog, birch, timothy, ragweed, wall pellitory, egg white, cow’s milk, peanut and shrimp). Atopy was defined as having detectable allergen- specific IgE level of ≥0.35 PAU/L. The cuff-off value for cord serum IgE was set at 0.5 IU/L and total serum IgE at 1 year of age was at 97 IU/L^{31 (link)}.

Liao S.L., Tsai M.H., Yao T.C., Hua M.C., Yeh K.W., Chiu C.Y., Su K.W., Huang S.Y., Kao C.C., Lai S.H, & Huang J.L. (2017). Caesarean Section is associated with reduced perinatal cytokine response, increased risk of bacterial colonization in the airway, and infantile wheezing. Scientific Reports, 7, 9053.

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Measuring Serum IgE Levels

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Total serum and allergen-specific serum IgE levels were examined as described in our previous study^{25 (link)}. Total serum IgE level was measured by ImmunoCAP (Phadia, Uppsala, Sweden), whereas specific IgE levels to Dermatophagoides pteronyssinus and Dermatophagoides farinae allergens were determined using a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia)^{26 (link)} and recorded as a continuous variable.

Chiu C.Y., Cheng M.L., Chiang M.H., Wang C.J., Tsai M.H, & Lin G. (2021). Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma. Scientific Reports, 11, 23407.

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Measurement of Serum IgE Levels

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The serum samples of enrolled subjects were collected at outpatient clinics in the morning and immediately stored at −80 °C in aliquots until required. The total serum and allergen-specific serum IgE levels were examined as described in our previous study [8 (link)]. The total serum IgE level was measured by ImmunoCAP (Phadia, Uppsala, Sweden), and the specific IgE levels to food (egg white and cow’s milk) and inhalant (Dermatophagoides pteronyssinus and Dermatophagoides farinae) allergens were determined using a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia) [9 (link)]. Allergen-specific IgE levels ≥ 0.35 kU/L was defined as positive and indicative of allergic sensitization [10 (link)].

Chiu C.Y., Cheng M.L., Chiang M.H., Wang C.J., Tsai M.H, & Lin G. (2020). Metabolomic Analysis Reveals Distinct Profiles in the Plasma and Urine Associated with IgE Reactions in Childhood Asthma. Journal of Clinical Medicine, 9(3), 887.

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IgE Measurement by ImmunoCAP Assay

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The serum level of total IgE was determined by ImmunoCAP (Phadia, Uppsala, Sweden). Specific IgE was determined by a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia) against the most common inhalant and food allergens (i.e. house dust mite, cat, dog, birch, timothy, ragweed, wall pellitory, egg white, cow's milk, peanut and shrimp) [21] (link).

Yao T.C., Tu Y.L., Chang S.W., Tsai H.J., Gu P.W., Ning H.C., Hua M.C., Liao S.L., Tsai M.H., Chiu C.Y., Lai S.H., Yeh K.W, & Huang J.L. (2014). Suboptimal Vitamin D Status in a Population-Based Study of Asian Children: Prevalence and Relation to Allergic Diseases and Atopy. PLoS ONE, 9(6), e99105.

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Serum IgE and Cytokine Levels

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Total serum and allergen-specific serum IgE levels were examined based on a previous study.15 (link) Total serum IgE level was measured by ImmunoCAP (Phadia), and specific IgE levels to food (egg protein, milk, peanut, soybean, wheat, buckwheat, cashew nut, pistachio nuts, filbert, beef, mutton, shrimp, crab, peach, apple, mango, and strawberry) and inhalant (Dermatophagoides pteronyssinus and Dermatophagoides farinae) allergens were determined using a commercial assay for IgE (ImmunoCAP Phadiatop Infant; Phadia). Serum cytokine IL-4 level was measured according to the manufacturer’s instructions.

Zou X.L., Wu J.J., Ye H.X., Feng D.Y., Meng P., Yang H.L., Wu W.B., Li H.T., He Z, & Zhang T.T. (2021). Associations Between Gut Microbiota and Asthma Endotypes: A Cross-Sectional Study in South China Based on Patients with Newly Diagnosed Asthma. Journal of Asthma and Allergy, 14, 981-992.

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Childhood Allergies: IgE Profiles

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Serum samples of children at different years of age were collected and measured. Simultaneous measurements were conducted for total IgE levels and specific IgE targeting the two most prevalent food allergens (egg white, milk), as well as inhalant allergens (D. pteronyssinus and D. farinae). Total serum IgE level was determined using ImmunoCAP (Phadia, Uppsala, Sweden), with IgE levels ≥100 kU/L indicating IgE sensitization.¹² (link) Allergen-specific IgE was examined by ImmunoCAP Phadiatop Infant, a commercially available IgE assay, with allergen sensitization defined as values ≥ 0.35 kU/L.¹³ (link)

Shen C.H., Chen C.B., Chiang M.H., Kuo C.N., Chung W.H., Lin Y.K, & Chiu C.Y. (2024). Vitamin D level is inversely related to allergen sensitization for risking atopic dermatitis in early childhood. The World Allergy Organization Journal, 17(4), 100890.

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