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Vinculin v4139

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Vinculin (V4139) is a cytoskeletal protein that is involved in the formation and regulation of cell-cell and cell-matrix junctions. It plays a role in the anchoring of the actin cytoskeleton to the plasma membrane. Vinculin is a commonly used marker for focal adhesions and adherens junctions.

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Lab products found in correlation

P erk, by Cell Signaling Technology (2 mentions) P akt thr308, by Cell Signaling Technology (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) Total erk, by Cell Signaling Technology (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) P creb, by Cell Signaling Technology (1 mentions) Fgfr1, by Cell Signaling Technology (1 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) β actin a2228, by Merck Group (1 mentions) Pfrs2, by Cell Signaling Technology (1 mentions) T erk, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Kras sc 30, by Santa Cruz Biotechnology (1 mentions)

2 protocols using vinculin v4139

1

Western Blot Antibodies for Signaling

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Antibodies used for Western blot analysis included the following: pERK (#9101), total ERK (#4695), pAKT-Ser473 (#9271), pAKT-Thr308 (#13038), total AKT (#2920), PTEN (#9559), PI3K-p110a (#4249) from Cell Signaling; Vinculin (V4139) from Sigma; BRAF (sc-5284) and KRAS (sc-517599) from Santa Cruz; HRAS (18295-1-AP) from ProteinTech; PI3K-p85 (06-195) from Millipore; Total RAS from ThermoScientific (#1862335). All antibodies were diluted to 1:1000.
Nigam A., Krishnamoorthy G., Chatila W., Berman K., Saqcena M., Walch H., Ho A., Schultz N., Fagin J, & Untch B. (2023). Cooperative Genomic Lesions in HRAS-Mutant Cancers Predict Resistance to Farnesyltransferase Inhibitors. Research Square.
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2

Immunoblot Analysis of Key Signaling Proteins

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The following antibodies were used for immunoblots at a dilution of 1:1000, except where indicated. HRAS (sc-520), NRAS (sc-31; 1:500), KRAS (sc-30; 1:500), FRS2 (sc-8318), CREB (sc-58) and GFP (sc-9996) were from Santa Cruz Biotechnology; pMEK S217/221 (#9121), tMEK (#9122), pERK (#9101), tERK (#4695), FGFR1 (#9740), pFRS2 (#3864), pAKT (#9271), EGFR (#4267), pEGFR (#3777) and pCREB (#9198) were from Cell Signaling Technology; β-Actin (A2228; 1:10000) and Vinculin (V4139) were from Sigma; and NF1 (A300-140A-M) was from Bethyl Laboratories.
Untch B.R., Anjos V.D., Garcia-Rendueles M.E., Knauf J.A., Krishnamoorthy G.P., Saqcena M., Bhanot U.K., Socci N.D., Ho A.L., Ghossein R, & Fagin J.A. (2018). Tipifarnib inhibits HRAS-driven dedifferentiated thyroid cancers. Cancer research, 78(16), 4642-4657.
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