The ROS detection reagent, a chloromethyl derivative of 2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA); MitoSOX Red Mitochondrial Superoxide Indicator (M36008); and MitoTracker Deep Red FM (M22426) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dihydroethidium was provided by MedChemExpress (Monmouth Junction, NJ, USA), and the pCT-COX8-GFP gene lentivirus for GFP-tagged mitochondria-specific HCEC was purchased from Vigene Biosciences (Rockville, MD, USA). Anti-Fis1 (10956-1-AP), Tom20 (66777-1-Ig), and MFF antibodies (17090-1-AP) were purchased from Proteintech (Rosemont, IL, USA). The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): DRP1 Rabbit mAb (8570s), AMPKα Antibody (2532), and Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (2535). Anti-LC3B antibody (ab51520) and Anti-SQSTM1/p62 antibody (ab91526) were purchased from Abcam (Cambridge, UK). Phospho-C2orf33 (Ser172, Ser146) Polyclonal Antibody (PA5-104614) was purchased from Thermo Fisher Scientific.
Phospho ampkα thr172 40h9 rabbit mab 2535
Manufactured by Cell Signaling Technology
Sourced in United States
The Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (2535) is a primary antibody that specifically recognizes the phosphorylated form of AMP-activated protein kinase alpha (AMPKα) at threonine 172. This antibody can be used to detect and quantify the phosphorylation status of AMPKα in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Mitotracker deep red fm, by Thermo Fisher Scientific (1 mentions)
Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions)
Pierce protease and phosphatase inhibitor mini tablet, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Roche (1 mentions)
2 protocols using phospho ampkα thr172 40h9 rabbit mab 2535
1
Mitochondrial Oxidative Stress Measurement
2
BARP Protein Stabilization by Metalloprotease Inhibitor
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Western blots were performed as described previously47 (link). To stabilize the BARP protein, 0.5 mM of the metalloprotease inhibitor bathophenanthroline disulphonic acid (Acros Organics, catalogue no. AC164050050) was added to each 48-h RBP6 induced cell line and incubated for 24 h. These cells were then harvested for Western blot analysis. Rabbit antiserum against RBP6, BARP, and mVSG397 as well as mouse antiserum against paraflagellar rod (PFR) or elongation factor 1-alpha (EF-1α, clone CBP-KK1 from Sigma-Aldrich) was used at a 1:1000 dilution. Either PFR or EF-1α was used as a loading control for each Western blot. Pierce Protease and Phosphatase Inhibitor Mini Tablets (Thermofisher, catalog no. A32959) was dissolved into the lysis buffer following manufacturer’s instruction and Western blots were probed with the rabbit polyclonal anti-p-AMPK (1:1000) antibody (Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535, Cell Signaling technologies). The horseradish peroxidase-conjugated secondary antibody (Roche) was used at a 1:5000 dilution.
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