Fluorescence imaging was carried out as previously reported (Nishigaki et al., 2004 (link)). Briefly, Fluo-4-labeled spermatozoa were adhered to glass coverslips coated with 50 Pg/ml poly-L-lysine solution (Sigma) and mounted into a micro incubator, PDMI-2 (Harvard Apparatus, Holliston, MA, USA) maintained at 14 °C. Fluorescence images were acquired using a Nikon DIAPHOT 300 inverted microscope with a Nikon Plan Apo 60X objective lens (1.4 NA) and a custom-built stroboscopic illumination system (Nishigaki et al., 2006 (link)) with a Chroma filter set (Ex, HQ470/40x; DC, 505DCXRU; Em, HQ510LP (Chroma Technology)). Images were acquired with a Quantix 57 camera (Photometrics Inc.) under the continuous (stream) acquisition mode.
505dcxru
Manufactured by Chroma Technology
The 505DCXRU is a lab equipment product. It is a compact, versatile power supply unit designed for use in various laboratory applications. The device provides stable and adjustable direct current (DC) output voltages and currents to power a range of laboratory equipment and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Hq510lp, by Chroma Technology (1 mentions)
Diaphot 300 inverted, by Nikon (1 mentions)
Poly l lysine solution, by Merck Group (1 mentions)
Pdmi 2, by Harvard Apparatus (1 mentions)
Dulbecco s pbs, by Thermo Fisher Scientific (1 mentions)
U lh75xeapo, by Olympus (1 mentions)
Andor ixon emccd camera, by Oxford Instruments (1 mentions)
Glass bottom dish, by Thermo Fisher Scientific (1 mentions)
2 protocols using 505dcxru
1
Fluorescence Imaging of Labeled Spermatozoa
2
Stochastic Insertion of Nanorods into Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The microscope setup was based on an Olympus IX71 inverted microscope equipped with a xenon lamp (75 W; U-LH75XEAPO, Olympus) and excitation filter (BP 470/40, Chroma Technology Corp). The excitation power was 2 mW at the image plane. The emission of the NPs was collected by a 60× objective lens (PlanApo 60×, NA = 1.45, oil immersion, Olympus) and passed through a dichroic mirror (505DCXRU, Chroma Technology Corp). Imaging was carried out with an Andor iXon EMCCD camera (Andor iXon). Two microliters of pcNRs in DMSO solution (~300 nM) were loaded to the glass-bottom dish (Thermo Fisher Scientific) where the self-spiking HEK293 cells were cultured. The pcNRs spontaneously inserted into cell membranes within 1 to 2 min. The pcNR-loading density estimated from the image was ~105 pcNRs per cell. After rapid shaking, the cell medium was changed with Dulbecco’s PBS (Life Technologies). The dish was then placed on the microscope. Fluorescence was recorded in a movie format for 9 s with a 30-ms integration per frame.
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