Embryos were isolated, fixed with 4% paraformaldehyde for 30 min to 2 h at 4 °C, embedded in paraffin, and sectioned at 5–6 μm with Leica RM2235. Sections were deparaffinized with ethanol of gradient concentration, then blocked in blocking solution (Zhongshan golden bridge) for 30 min at room temperature, followed by incubation with primary antibodies overnight at 4 °C. After 3 washes (3 min each) in PBS, sections were incubated with corresponding secondary antibodies (Zhongshan golden bridge) for 30 min at room temperature. After 3 washes in PBS, sections were stained with DendronFluor TSA (Histova, NEON 4-color IHC Kit for FFPE, NEFP450, 1:100, 20–60 s). The primary and secondary antibodies were thoroughly eluted by heating the slides in citrate buffer (pH 6.0) for 20 min at 95 °C using microwave. In a serial fashion, each antigen was labeled by distinct fluorophores. After all the antibodies were detected sequentially, the slices were finally stained with DAPI. Images were collected by confocal microscope (Nikon Ti-E A1/ ZEISS LSM 880). The primary antibodies were as follows: CD31 (BD Biosciences), CD44 (BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).
Ti e a1 lsm 880
Manufactured by Zeiss
The Ti-E A1/ ZEISS LSM 880 is a high-performance microscope system designed for advanced imaging applications. It combines the Ti-E A1 inverted microscope with the ZEISS LSM 880 laser scanning confocal microscope. The system offers high-resolution imaging capabilities and is suitable for a wide range of research and analysis tasks.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ti e a1 lsm 880
1
Multicolor Immunohistochemistry for Embryo Analysis
2
Multicolor Immunohistochemistry of Embryonic Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Embryos were isolated, fixed with 4% paraformaldehyde for 30 minutes to 2 hours at 4°C, embedded in paraffin, and sectioned at 5-6 μ m with Leica RM2235. Sections were deparaffinized with ethanol of gradient concentration, then blocked in blocking solution (Zhongshan golden bridge) for 30 minutes at room temperature, followed by incubation with primary antibodies overnight at 4°C. After 3 washes (3 minutes each) in PBS, sections were incubated with corresponding secondary antibodies (Zhongshan golden bridge) for 30 minutes at room temperature. After 3 washes in PBS, sections were stained with DendronFluor TSA (Histova, NEON 4-color IHC Kit for FFPE, NEFP450, 1:100, 20-60-sec).
The primary and secondary antibodies were thoroughly eluted by heating the slides in citrate buffer (pH 6.0) for 10 minutes at 95°C using microwave. In a serial fashion, each antigen was labeled by distinct fluorophores. After all the antibodies were detected sequentially, the slices were finally stained with DAPI. Images were collected by confocal microscope (Nikon Ti-E A1/ ZEISS LSM 880). The primary antibodies were as follows: CD31 (BD Biosciences), CD44
(BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).
The primary and secondary antibodies were thoroughly eluted by heating the slides in citrate buffer (pH 6.0) for 10 minutes at 95°C using microwave. In a serial fashion, each antigen was labeled by distinct fluorophores. After all the antibodies were detected sequentially, the slices were finally stained with DAPI. Images were collected by confocal microscope (Nikon Ti-E A1/ ZEISS LSM 880). The primary antibodies were as follows: CD31 (BD Biosciences), CD44
(BD Biosciences), Endomucin (eBioscience), GFP (Cell Signaling), and Runx1 (Abcam).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!