Log In Sign up
The largest database of trusted experimental protocols

The DDAOG is a laboratory instrument designed for the detection and quantification of specific molecules or analytes in a sample. It utilizes a combination of fluorescence detection and enzymatic reactions to provide accurate and sensitive measurements. The core function of the DDAOG is to enable researchers and scientists to analyze and quantify the presence of target compounds in their research or testing applications.

Automatically generated - may contain errors

Lab products found in correlation

In cell analyzer 2000, by GE Healthcare (3 mentions) Bafilomycin a1, by Merck Group (1 mentions) X gal, by Roche (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions)

6 protocols using ddaog

1

Visualizing Intracellular Organelle Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were sequentially cultured with 100 nM Bafilomycin A1 (Sigma) and 100 μM DDAOG (Thermo), 1 and 2 h respectively at 37 °C as previously described41 (link). Cells were then fixed 15 min 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 10 min. Images were acquired the same day at 647 nM exposure.
Ferreira-Gonzalez S., Lu W.Y., Raven A., Dwyer B., Man T.Y., O’Duibhir E., Lewis P.J., Campana L., Kendall T.J., Bird T.G., Tarrats N., Acosta J.C., Boulter L, & Forbes S.J. (2018). Paracrine cellular senescence exacerbates biliary injury and impairs regeneration. Nature Communications, 9, 1020.
+ Open protocol
+ Expand
2

Senescent Cell Detection via DDAOG Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Senescent cells (SCs) were detected used the fluorescent senescence-associated-β-galactosidase assay (19 (link), 20 (link)). Callus cells or cultured CaMPCs were treated with 100 μM 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-galactopyranoside (DDAOG) Thermo Fisher Scientific, cat#: D-6488) for 50 minutes and subjected to flow cytometry for 7-hydroxy-9H(I,3-dichloro-9,9-dimethylacridin-2-one) signal.
Liu J., Lin X., McDavid A., Yang Y., Zhang H., Boyce B.F, & Xing L. (2023). Molecular signatures distinguish senescent cells from inflammatory cells in aged mouse callus stromal cells. Frontiers in Endocrinology, 14, 1090049.
+ Open protocol
+ Expand
3

DDAOG-Induced Cell Fixation and Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown on 96‐well plates. After 8 days, cells were treated with 100 μM DDAOG (D‐6488, Thermo Fisher Scientific) 2 hr before fixation with 4% PFA for 15 min. Nuclei were stained with DAPI (1 μg/ml) for 15 min and images acquired the same day using a IN Cell Analyzer 2000 (GE Healthcare).
Guerrero A., Guiho R., Herranz N., Uren A., Withers D.J., Martínez‐Barbera J.P., Tietze L.F, & Gil J. (2020). Galactose‐modified duocarmycin prodrugs as senolytics. Aging Cell, 19(4), e13133.
+ Open protocol
+ Expand
4

Senescence-Associated β-Galactosidase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown on 96-well plates and incubated with DDAOG (D-6488, Life Technologies) for 2 hours. Cells were then fixed with 4% PFA for 15 min and nuclei was stained with 1 μg/ml DAPI. Images were taken using high-throughput fluorescent microscope IN Cell Analyzer 2000 (GE Healthcare) with a 20x objective. The percentage of SA-β-Gal positive cells was estimated using the INCell Investigator 2.7.3 software based on differences on cell intensity over an arbitrary threshold.
Guerrero A., Herranz N., Sun B., Wagner V., Gallage S., Guiho R., Wolter K., Pombo J., Irvine E.E., Innes A.J., Birch J., Glegola J., Manshaei S., Heide D., Dharmalingam G., Harbig J., Olona A., Behmoaras J., Dauch D., Uren A.G., Zender L., Vernia S., Martínez-Barbera J.P., Heikenwalder M., Withers D.J, & Gil J. (2019). Cardiac glycosides are broad-spectrum senolytics. Nature metabolism, 1(11), 1074-1088.
+ Open protocol
+ Expand
5

Neutrophil Characterization in Tumor Microenvironment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse anti-HIS48 antibody (Ab) (Santa Cruz) was used to characterize tumor-infiltrating rat neutrophils. Tumor cells were identified using a mouse anti-cytokeratin antibody (DakoCytomation). The following Abs were also used: a rabbit anti-iNOS Ab (BD Transduction Laboratories) and a mouse anti-M30 fragment (Roche). Secondary anti-mouse or anti-rabbit Alexa Fluor 568 or 488 conjugates antibodies were purchased from Molecular Probes, Invitrogen. X-Gal (Roche) and DDAOG (Life Technologies) were used to characterize Senescence-Associated-β-Galactosidase (SA-β-Gal) activity into the cells. The lipid A analog OM-174 was kindly provided by OM Pharma (Meyrin, Switzerland).
Seignez C., Martin A., Rollet C.E., Racoeur C., Scagliarini A., Jeannin J.F., Bettaieb A, & Paul C. (2014). Senescence of tumor cells induced by oxaliplatin increases the efficiency of a lipid A immunotherapy via the recruitment of neutrophils. Oncotarget, 5(22), 11442-11451.
+ Open protocol
+ Expand
6

Senescence-Associated β-Galactosidase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown on 96-well plates and incubated with DDAOG (D-6488, Life Technologies) for 2 hours. Cells were then fixed with 4% PFA for 15 min and nuclei was stained with 1 μg/ml DAPI. Images were taken using high-throughput fluorescent microscope IN Cell Analyzer 2000 (GE Healthcare) with a 20x objective. The percentage of SA-β-Gal positive cells was estimated using the INCell Investigator 2.7.3 software based on differences on cell intensity over an arbitrary threshold.
Guerrero A., Herranz N., Sun B., Wagner V., Gallage S., Guiho R., Wolter K., Pombo J., Irvine E.E., Innes A.J., Birch J., Glegola J., Manshaei S., Heide D., Dharmalingam G., Harbig J., Olona A., Behmoaras J., Dauch D., Uren A.G., Zender L., Vernia S., Martínez-Barbera J.P., Heikenwalder M., Withers D.J, & Gil J. (2019). Cardiac glycosides are broad-spectrum senolytics. Nature metabolism, 1(11), 1074-1088.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!