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4 pregnen 11β 21 diol 3 20 dione 21 hemisuccinate

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4-pregnen-11β 21-DIOL-3 20-DIONE 21-hemisuccinate is a chemical compound used in biochemical and pharmaceutical research applications. It is a derivative of the steroid hormone progesterone. The product serves as a precursor or intermediate for the synthesis of other steroid-based molecules.

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Lab products found in correlation

Spironolactone, by Merck Group (2 mentions) Mifepristone, by Merck Group (2 mentions) Ly341495, by Bio-Techne (1 mentions) Corticosterone hemisuccinate, by Steraloids (1 mentions) Ly379268, by Bio-Techne (1 mentions)

5 protocols using 4 pregnen 11β 21 diol 3 20 dione 21 hemisuccinate

1

Corticosterone and Antagonist Effects

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Male C57BL/6J mice were single housed 4 days prior to the experiment and their daily water intake was monitored. On the experimental day, mice were treated overnight (14 h) with either CORT (0.1 mg/ml in drinking water resulting in a ~25 mg/kg average dose based on the initially determined fluid intake; 4-pregnen-11β 21-DIOL-3 20-DIONE 21-hemisuccinate, #Q1562-000, Steraloids), RU486 (0.05 mg/ml in 0.5% EtOH resulting in a ~10 mg/kg average dose based on the initially determined fluid intake; Mifepristone, #475838, Sigma-Aldrich) or Spironolactone (0.124 mg/ml in 0.4% EtOH resulting in a ~20 mg/kg average dose based on the initially determined fluid intake; #S3378, Sigma-Aldrich). Control animals received their respective vehicle solutions. The next morning all mice were sacrificed by decapitation following quick anesthesia by isoflurane. Brains were removed, snap-frozen in isopentane at −40°C, and stored at −80°C until further processing. Overnight fluid intake did not differ between treatment groups.
Hartmann J., Bajaj T., Klengel C., Chatzinakos C., Ebert T., Dedic N., McCullough K.M., Lardenoije R., Joëls M., Meijer O.C., McCann K.E., Dudek S.M., Sarabdjitsingh R.A., Daskalakis N.P., Klengel T., Gassen N.C., Schmidt M.V, & Ressler K.J. (2021). Mineralocorticoid receptors dampen glucocorticoid receptor sensitivity to stress via regulation of FKBP5. Cell reports, 35(9), 109185.
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2

Chronic Corticosterone Exposure in Mice

Cited 2 times
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4-pregnen-11β 21-DIOL-3 20-DIONE 21-hemisuccinate (Steraloids) was dissolved in tap water (25 μg/ml free base; Gourley et al., 2008a (link),b (link); Barfield et al., 2017 (link)). CORT-exposed mice were given CORT-infused drinking water, while control mice consumed tap water.
Water bottles were weighed daily, and mice weighed every other day, allowing us to calculate the amount of liquid displaced/total weight of all mice in the cage. With this value and the known concentration of CORT in hand, we were then able to calculate the approximate dose of CORT ingested by a mouse in any given cage (5–9 mg/kg/day). Every 3 days, water bottles were refilled with freshwater or newly prepared CORT solution.
Mice were exposed to CORT or water from postnatal day (P) 31–56 to span most of the rodent adolescent period (Spear, 2000 (link)). Mice were euthanized before CORT exposure (P31, baseline), during CORT exposure (P42, P49, P56), or at P70 following a 2-week washout period without CORT (see the timeline in Figure 1A).
Barfield E.T., Sequeira M.K., Parsons R.G, & Gourley S.L. (2020). Morphological Responses of Excitatory Prelimbic and Orbitofrontal Cortical Neurons to Excess Corticosterone in Adolescence and Acute Stress in Adulthood. Frontiers in Neuroanatomy, 14, 45.
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3

Corticosterone Exposure in Mice

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4-pregnen-11β 21-DIOL-3 20-DIONE 21-hemisuccinate (Steraloids) was dissolved in tap water (25 μg/mL free base). CORT-exposed mice were given CORT in place of normal drinking water, while control mice consumed tap water. Water bottles were weighed daily, and mice weighed every other day to calculate average doses (~5–9 mg/kg/day) of CORT. Every 3 days, water bottles were refilled with fresh tap water or newly prepared CORT solution. Mice were exposed to CORT or water from postnatal day (P) 31–42 or P56-67, corresponding to early adolescence and adulthood in rodents (Spear, 2000 (link)). After a washout period of at least 2 weeks, instrumental conditioning began. Timelines are provided in the respective figures.
Importantly, this procedure was validated for use in adolescent mice in our prior report (Barfield et al., 2017 ), confirming that, as in adults, it elevates blood serum CORT to levels comparable to those following forced swim stress and causes adrenal and thymus gland atrophy that recovers when exogenous CORT is removed. Thus, the procedure allows us to isolate the neurobehavioral consequences of stress-like CORT levels (see also Gourley et al., 2012 (link)).
Barfield E.T, & Gourley S.L. (2019). Glucocorticoid-sensitive ventral hippocampal-orbitofrontal cortical connections support goal-directed action – Curt Richter Award Paper 2019. Psychoneuroendocrinology, 110, 104436.
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4

Alcohol and Corticosterone Administration Protocol

Cited 3 times
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Alcohol (95%) was diluted in distilled water to a concentration of 20% (v/v) and administered IG, with volumes varied by weight to obtain the desired dose. Corticosterone hemisuccinate (4-pregnen-11β, 21-DIOL-3, 20-DIONE 21-hemisuccinate; Steraloids, Newport, RI) was dissolved in tap water by addition of NaOH and neutralized with HCl, to a final pH of 7.0–7.4 (Besheer et al., 2012 (link), 2013 (link); Gourley & Taylor, 2009 (link)). (1R,4R,5S,6R)-4-Amino-2-oxabicyclo[3.1.0]hexane-4,6-dicarboxylic acid disodium salt (LY379268; Tocris, Ellisville, Missouri), a highly selective (IC50~10 nM in the presence of LY341495; Imre, 2007 (link)) Group II (mGluR2/3) agonist, and (2S)-2-Amino-2-[(1S,2S)-2-carboxycycloprop-1-yl]-3-(xanth-9-yl) propanoic acid disodium salt (LY341495; Tocris, Ellisville, Missouri), a highly potent (Kd~0.8 nM) and selective Group II (mGluR2/3) antagonist, were dissolved in saline and injected at a volume of 1 mL/kg. The doses of the mGluR2/3 compounds were selected based on pilot studies in our lab and on previous work (Bäckstrom & Hyytiä, 2005 (link); Cannady et al., 2011 (link); Liechti et al., 2007 (link); Monn et al., 1999 (link); Sidhpura et al., 2010 (link); Wright, Arnold, Wheeler, Ornstein, & Schoepp, 2001 (link)).
Jaramillo A.A., Randall P.A., Frisbee S., Fisher K.R, & Besheer J. (2015). Activation of mGluR2/3 following stress hormone exposure restores sensitivity to alcohol in rats. Alcohol (Fayetteville, N.Y.), 49(6), 525-532.
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5

Corticosterone and Antagonist Effects

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Male C57BL/6J mice were single housed 4 days prior to the experiment and their daily water intake was monitored. On the experimental day, mice were treated overnight (14 h) with either CORT (0.1 mg/ml in drinking water resulting in a ~25 mg/kg average dose based on the initially determined fluid intake; 4-pregnen-11β 21-DIOL-3 20-DIONE 21-hemisuccinate, #Q1562-000, Steraloids), RU486 (0.05 mg/ml in 0.5% EtOH resulting in a ~10 mg/kg average dose based on the initially determined fluid intake; Mifepristone, #475838, Sigma-Aldrich) or Spironolactone (0.124 mg/ml in 0.4% EtOH resulting in a ~20 mg/kg average dose based on the initially determined fluid intake; #S3378, Sigma-Aldrich). Control animals received their respective vehicle solutions. The next morning all mice were sacrificed by decapitation following quick anesthesia by isoflurane. Brains were removed, snap-frozen in isopentane at −40°C, and stored at −80°C until further processing. Overnight fluid intake did not differ between treatment groups.
Hartmann J., Bajaj T., Klengel C., Chatzinakos C., Ebert T., Dedic N., McCullough K.M., Lardenoije R., Joëls M., Meijer O.C., McCann K.E., Dudek S.M., Sarabdjitsingh R.A., Daskalakis N.P., Klengel T., Gassen N.C., Schmidt M.V, & Ressler K.J. (2021). Mineralocorticoid receptors dampen glucocorticoid receptor sensitivity to stress via regulation of FKBP5. Cell reports, 35(9), 109185.
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