Seqman program of software version 7

The nucleotide and deduced amino acid (AA) sequences of PPVs were assembled using the SeqMan program of DNAstar software version 7.0 (DNASTAR Inc., Madison, WI, USA) to determine sequence homology and genetic variations. The phylogenetic analysis is based on the nucleotide sequence by the distance-based neighbor-joining method using the iTOL v6 with bootstrap analysis of 1000 replicates (https://itol.embl.de/, accessed on 26 November 2021). All reference sequence information is listed in Supplementary Tables S1–S6 (the nucleotide database is recorded as of 2021).

The obtained full-length genomic sequences of SCN17 were assembled using the SeqMan program of DNAstar software, version 7.0 (DNASTAR Inc., Madison, WI, USA). Further, the sequences of PRRSV genomes, ORFs, and deduced proteins were analyzed by the EditSeq and MegAlign programs of DNAstar (DNASTAR Inc., Madison, WI, USA). Next, phylogenetic analysis was performed based on the nucleotide sequences, generated by the distance-based neighbor-joining method using the Molecular Evolutionary Genetics Analysis 6 (MEGA 6) software (www. megasoftware.net). Finally, the bootstrap values of the phylogenetic tree were evaluated with 1000 replicates.