Images of hematoxylin and eosin stained muscle sections were captured from a Nikon 800 microscope with 10× or 20× Plan Apo objectives and Canon EOS T3 camera using EOS Utility image acquisition software. Fluorescent images of muscle sections and single myofibers were wither captured using Leica SP5 confocal equipped with 40×/1.25 Plan Apo oil objectives using Leica image acquisition software, or a Zeiss Axioskope equipped with a 40×/0.5 Plan Apo oil objective and Axiocam camera using Zeiss image acquisition software. Identical exposure times were used and images were processed and scored with blinding using ImageJ64. If necessary, brightness and contrast were adjusted for an entire experimental image set. For quantification of polarized cell markers (Par3, m-cadherin, pp38), all authors individually scored each set of images with blinding using ImageJ64, only those that were agreed upon by all were included. For the rest, Imaris (Bitplane) was used for three-dimensional rendering of fluorescence data for quantification of polarization. A subset was also randomly selected for quantitative analysis via Imaris to confirm authors’ scoring. Cell number, fiber diameter, fiber number, and fiber cross sectional area were determined using ImageJ64 or Fiji using images of a micrometer (VWR) taken under the same magnifications as the sample images as references for imaging field sizes.
Eos t3
Manufactured by Canon
The EOS T3 is a digital single-lens reflex (DSLR) camera manufactured by Canon. It features a 12.2-megapixel image sensor, DIGIC 4 image processor, and a 2.7-inch LCD screen. The EOS T3 is capable of capturing high-quality images and Full HD 1080p video.
Automatically generated - may contain errors
4 protocols using eos t3
1
Quantitative Analysis of Muscle Histology
2
Quantitative Analysis of Muscle Histology
3
Muscle Fiber Imaging and Analysis
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The H&E‐stained TA muscle sections were imaged with a Nikon 800 microscope using 20× Plan Apo objectives and Canon EOS T3 camera using EOS Utility acquisition software. Fluorescent imaging of TA muscle sections was captured using a Nikon Eclipse E800 microscope with 20×/0.50 Plan Fluor oil objectives and Hamamatsu digital camera C11440 using MetaMorph Microscopy Automation and Image Analysis Software. For fluorescent imaging of myoblasts in vitro, images were captured using a Nikon Ti2 inverted microscope controlled by the Element software with 20×/0.50 Plan Fluor long distance objective. For quantification of muscle fiber number, diameter, cell density, and differentiation/fusion indices, images were analyzed in ImageJ (v 1.44p, NIH, Bethesda), an image processing program. Pixel to μm ratios were determined by imaging a micrometer using the same settings for each set of data, and used to size the scale bars.
4
Andean Cloud Forest Insect Sampling
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Specimens of the new taxa were sampled in Andean Cloud Forest at Wayquecha Biological Station (Cusco Region) by Malaise traps (Fig. 1) at elevations of 2692 m and 2865 m a.s.l. Images were acquired using a Leica M205 C stereo microscope and stacking software LAS ver. 4 at increments of 20-50 steps, and with a Canon EOS T3 digital camera attached to an Infinity K-2 long-distance microscopic lens at the MUSM. The morphological terminology follows Gauld (1991) .
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