Integrin α6

Manufactured by Merck Group

Sourced in United States

Integrin α6 is a cellular receptor protein that mediates cell-cell and cell-extracellular matrix interactions. It functions as a subunit of the integrin heterodimer and is involved in various biological processes, including cell adhesion, migration, and signaling.

Automatically generated - may contain errors

Lab products found in correlation

Integrin β1, by Merck Group (2 mentions) Alexa fluor 568, by Thermo Fisher Scientific (2 mentions) Integrin β1, by Cell Signaling Technology (2 mentions) Matrigel, by Corning (2 mentions) Gapdh, by Thermo Fisher Scientific (2 mentions) Integrin α5, by Abcam (2 mentions) Hif 2α, by Abcam (2 mentions) β actin, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Vinculin, by Abcam (2 mentions) Hif 1α, by Cell Signaling Technology (2 mentions) Active integrin β1 clone huts 4, by Merck Group (2 mentions) Lsm 5 pascal, by Zeiss (2 mentions) Facscalibur, by BD (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions) Hoechst, by Merck Group (2 mentions) αvβ3 integrin, by BioLegend (2 mentions) Chamber slide, by Thermo Fisher Scientific (2 mentions) α4 integrin, by Merck Group (2 mentions)

Close spelling variants of this product

Anti-α6 integrin (2 citations)

8 protocols using integrin α6

Characterization of Pluripotent Stem Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the end of the differentiation period, the cells of each group were fixed with 4% paraformaldehyde for 30 min. Then, the cells were washed with 3x PBS. Next, the cells were blocked with 10% bovine serum albumin (BSA)-PBS at 37°C for 30 min and specific antigen (1:200 anti-Nanog, 1:200 anti-SSEA-1, 1:200 anti-Cvh, 1:250 anti-C-kit, 1:200 integrin α6, and 1:200 integrin β1; Millipore, Inc., Billerica, MA, USA) for one night at -4°C. Following a wash with 3x Phosphate Buffered Saline Tween (PBST), fluorescein isothiocyanate (FITC)- or PE-labeled goat anti-mouse immunoglobulin M (Bioss, China) was added and the incubation was continued at 37°C for 2 hours. The cells were then incubated with DAPI staining solution for 3 min at 37°C. After washing again with PBST, cells were observed under an inverted fluorescence microscope.

Zhang Y., Wang Y., Zuo Q., Wang X., Li D., Tang B, & Li B. (2016). Selection of the Inducer for the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells In Vitro. PLoS ONE, 11(10), e0164664.

+ Open protocol

+ Expand

Visualizing Extracellular Matrix Structure

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Click-iT® EdU Alexa Fluor® 488 Imaging Kit and Alexa Fluor® 594 phalloidin were from Invitrogen. Matrigel (lrECM) and Type I collagen were from BD Bioscience. ShP4HA2 plasmids were purchased from Sigma. 1, 4-DPCA was purchased from Cayman Chemical. Masson’s trichrome stain kit was purchased from Polysciences, Inc. The following antibodies were obtained as indicated: integrin α6 (Millipore); collagen I (Abcam); collagen IV (Abcam); P4HA2 (Santa Cruz); tubulin (Millipore).

Xiong G., Deng L., Zhu J., Rychahou P.G, & Xu R. (2014). Prolyl-4-hydroxylase α subunit 2 promotes breast cancer progression and metastasis by regulating collagen deposition. BMC Cancer, 14, 1.

+ Open protocol

+ Expand

Visualizing brain angiogenesis in GBM

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human GBM and normal brain tissue samples were snap frozen. Intracranial xenografts were fixed in 4% paraformaldehyde, incubated overnight in 30% sucrose and embedded in OCT. Samples were sectioned at a thickness of 10µm. Blood vessels were visualized by Von Willebrand Factor (Abcam) staining. Sections were additionally stained for CD36 (Novus), integrin α6 (Millipore), CD133 (Miltenyi), and/or Iba1 (Wako). Age-matched wild-type and CD36 knockout mouse brains were prepared in a similar manner as described above and stained with antibodies against proliferating cell nuclear antigen (Abcam) and phospho histone H3 (Millipore). Analysis between wild-type and CD36 knockout mice was done based on 3 different anatomical regions from 3 separate mice. For all immunofluorescence analysis, nuclei were counterstained using 4',6-diamidino-2-phenylindole (Dapi) and images were taken using a Leica SP-5 confocal microscope as previously described [27 (link)].

Hale J.S., Otvos B., Sinyuk M., Alvarado A.G., Hitomi M., Stoltz K., Wu Q., Flavahan W., Levison B., Johansen M.L., Schmitt D., Neltner J.M., Huang P., Ren B., Sloan A.E., Silverstein R.L., Gladson C.L., DiDonato J.A., Brown J.M., McIntyre T., Hazen S.L., Horbinski C., Rich J.N, & Lathia J.D. (2014). Cancer stem cell-specific scavenger receptor CD36 drives glioblastoma progression. Stem cells (Dayton, Ohio), 32(7), 1746-1758.

+ Open protocol

+ Expand

Stem Cell Differentiation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s modified eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco (Carlsbad, CA, U.S.A.). Human stem cell factor, basic fibroblast growth factor, retinoic acid (RA), and murine leukemia inhibitory factor were acquired from Sigma-Aldrich (Saint Louis, MO, U.S.A.). TRNzol, FastQuant-RT kit, and SuperReal premix color kit were obtained from TIANGEN (Beijing, China). Fugen was from Promega (Madison, WI, U.S.A.). Antibodies specific to the following proteins were integrinα6 (Millipore, temecula, CA, U.S.A.; dilution ratio 1:100), integrin β1 (Millipore, temecula, CA, U.S.A.; no. MAB1378; dilution ratio 1:100), goat anti-Rat IgG (Proteintech, Chicago, Illinois U.S.A.; no. SA00003-11; [FITC] labeled; dilution ratio, 1:100), and goat anti-rabbit IgG (Proteintech, Chicago, Illinois, U.S.A.; no. SA00008-9; [PE] labeled; dilution ratio, 1:100).

Zhang C., Wang F., Zuo Q., Sun C., Jin J., Li T., Wang M., Zhao R., Yu X., Sun H., Zhang Y., Chen G, & Li B. (2018). Cped1 promotes chicken SSCs formation with the aid of histone acetylation and transcription factor Sox2. Bioscience Reports, 38(5), BSR20180707.

+ Open protocol

+ Expand

Antibodies for Immunoblot and Immunocytochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for immunoblotting and immunocytochemistry: GAPDH (Invitrogen, #437000), HIF1-α (Cell Signaling Technology, #14179S), HIF2-α (Abcam, #ab207607), Fibronectin (IB) (Abcam, #ab23750), Fibronectin (ICC) (SCBT, #IST-9), β-Actin (Invitrogen, #MA1–140), Vinculin (Abcam, #129002), Integrin-α6 (Millipore Sigma #MABT356), Integrin-α5 (Abcam, #ab150361), Integrin-β1 (Cell Signaling Technology, #96996) Alexa Fluor 568 (Invitrogen #A10037) and Alexa Fluor 488 (Invitrogen, #A11001). For flowcytometry, Active Integrin-β1 clone HUTS-4 (Millipore Sigma, #MAB2079Z) was used. Matrigel^™ was purchased from Corning. Protein concentration in lots used varied between 10–11.2 mg/ml with an endotoxin level of <1.5.

Magdaleno C., House T., Pawar J.S., Carvalho S., Rajasekaran N, & Varadaraj A. (2021). Fibronectin assembly regulates lumen formation in breast acini. Journal of Cellular Biochemistry, 122(5), 524-537.

+ Open protocol

+ Expand

Antibody panel for cell analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for immunoblotting and immunocytochemistry: GAPDH (#437000; Invitrogen), HIF1‐α (#14179S; Cell Signaling Technology), HIF2‐α (#ab207607; Abcam), FN (IB) (#ab23750; Abcam), FN (ICC) (#IST‐9; SCBT), β‐actin (#MA1‐140; Invitrogen), Vinculin (#129002; Abcam), Integrin‐α6 (#MABT356; Millipore Sigma), Integrin‐α5 (#ab150361; Abcam), Integrin‐β1 (#96996; Cell Signaling Technology) Alexa Fluor 568 (#A10037; Invitrogen), and Alexa Fluor 488 (#A11001; Invitrogen). For flowcytometry, Active Integrin‐β1 clone HUTS‐4 (#MAB2079Z; Millipore Sigma) was used. Matrigel™ was purchased from Corning. Protein concentration in lots used varied between 10 and 11.2 mg/ml with an endotoxin level of less than 1.5.

+ Open protocol

+ Expand

Tracking EV Uptake in GECs and Podocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

We cultured GECs and podocytes on six-well plates or chamber slides (Thermo Scientific, Waltham, MA, USA). We incubated cells with PKH26-labelled EVs for 1 h, and then cells seeded on chamber slides were fixed with paraformaldehyde (Sigma Aldrich), nuclei were counterstained in blue by 2.5 μg/mL Hoechst (Sigma Aldrich), evaluated by confocal microscopy (Zeiss LSM 5 PASCAL, Jena, Germany). Cells cultured on six-well plates were detached by EDTA (Sigma) and analyzed by FACS (FACS Calibur, Becton Dickinson, Franklin Lakes, NJ, USA). In selected experiments, PKH26-labelled EVs were pre-incubated with 1 μg/mL of different antibodies directed to block the binding to αVβ-3 integrin (Biolegend, San Diego, CA, USA), α4-integrin, α6-integrin (Chemicon, Temecula, CA), CD29 or L-selectin (Becton Dickinson).

Medica D., Franzin R., Stasi A., Castellano G., Migliori M., Panichi V., Figliolini F., Gesualdo L., Camussi G, & Cantaluppi V. (2021). Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury. Cells, 10(7), 1675.

+ Open protocol

+ Expand

Internalization of EVs by GECs and Podocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

We cultured GECs and podocytes on 6-well plates or chamber slides (Thermo Scientific, Waltham, MA, USA). We incubated cells with PKH26-labelled EVs for 1 h on cells seeded on chamber slides were fixed with paraformaldehyde (Sigma Aldrich), nuclei were counterstained in blue by 2.5 μg/mL Hoechst (Sigma Aldrich), evaluated by confocal microscopy (Zeiss LSM 5 PASCAL, Jena, Germany). Cells cultured on 6-well plates were detached by EDTA (Sigma) and analyzed by FACS (FACS Calibur, Becton Dickinson, Franklin Lakes, NJ, USA). In selected experiments, PKH26-labelled EVs were pre-incubated with 1 μg/mL of different antibodies directed to block the binding to αVβ3integrin (Biolegend, San Diego, CA, USA), α4-integrin, α6-integrin (Chemicon, Temecula, CA), CD29 or L-selectin (Becton Dickinson).

Medica D., Cantaluppi V., Franzin R., Stasi A., Castellano G., Migliori M., Panichi V., & Camussi G. (2020). Extracellular Vesicles derived from Endothelial Progenitor Cells inhibit complement- and cytokine-mediated injury of renal glomerular endothelial cells and podocytes.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!