Invia raman imaging microscope

Manufactured by Renishaw

The Invia Raman-imaging microscope is a laboratory instrument designed for Raman spectroscopy and imaging. It provides high-resolution spatial and spectral analysis of samples. The system combines a confocal microscope with a spectrometer to capture detailed chemical and structural information about the sample.

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3 protocols using invia raman imaging microscope

Luminescence Spectra Analysis Under Pressure

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Luminescence spectra were measured with a Renishaw Invia Raman-imaging microscope equipped with a Peltier-cooled CCD camera. Excitation sources for the luminescence experiments were a 488 nm argon-ion laser and a 514 nm diode laser. The microscope was used to focus light onto a sample spot of approximately 1 µm in diameter and to collect the scattered light. Pressure-dependent measurements on solid samples in Nujol were made with a DAC (High-Pressure Diamond Optics). The ruby R1 line method (Piermarini et al., 1975 ▸ ) was used to calibrate the hydrostatic pressure inside the gasketed cell. All pressure-induced phenomena reported in this work are reversible upon gradual release of external pressure.

Woodall C.H., Christensen J., Skelton J.M., Hatcher L.E., Parlett A., Raithby P.R., Walsh A., Parker S.C., Beavers C.M., Teat S.J., Intissar M., Reber C, & Allan D.R. (2016). Observation of a re-entrant phase transition in the molecular complex tris(μ2-3,5-diisopropyl-1,2,4-triazolato-κ2 N 1:N 2)trigold(I) under high pressure. IUCrJ, 3(Pt 5), 367-376.

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Raman and Luminescence Spectroscopy of Solid Samples

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Raman and luminescence spectra were measured with a Renishaw Invia Raman imaging microscope equipped with a Peltier-cooled CCD camera. Excitation sources were a 488 nm argon ion laser and 514 nm diode laser for the luminescence experiments and a 785 nm diode laser for the Raman experiments. The microscope was used to focus light onto a spot of approximately 1 μm in diameter and to collect the scattered light. Low-temperature Raman experiments were obtained by coupling a Linkam coldfinger cryostat to the apparatus with liquid nitrogen used as coolant. A Janis closed cycle helium cryostat was used for low-temperature luminescence experiments. Pressure-dependent measurements on solid samples in nujol were made with a diamond-anvil cell (DAC, High-Pressure Diamond Optics). The ruby R1 line method12 was used to calibrate the hydrostatic pressure inside the gasketed cell. All pressure-induced phenomena reported here are reversible upon gradual release of external pressure.

Woodall C.H., Fuertes S., Beavers C.M., Hatcher L.E., Parlett A., Shepherd H.J., Christensen J., Teat S.J., Intissar M., Rodrigue-Witchel A., Suffren Y., Reber C., Hendon C.H., Tiana D., Walsh A, & Raithby P.R. (2014). Tunable Trimers: Using Temperature and Pressure to Control Luminescent Emission in Gold(I) Pyrazolate-Based Trimers. Chemistry (Weinheim an Der Bergstrasse, Germany), 20(51), 16933-16942.

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In Vivo and Ex Vivo Raman Imaging of Tumors

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Raman imaging was performed on a Renishaw InVia Raman imaging microscope, via the Wire software v4 interface. The mice were imaged twice, once through the intact skin and once after surgical exposure of the tumor area, with alternating order between control and treatment groups. One animal at a time was fastened on a platform and mounted on the microscope’s mechanized stage. Prior to each Raman scan, the focal plane was selected to intersect the tumors using white-light parfocal optics, and a widefield image was acquired. The tumor areas were selected, and scanned with the Raman laser (wavelength 785 nm, objective ×5, power 161 mW, acquisition 0.5 s, resolution 300 µm). The platform was then removed from the microscope, the tumors exposed surgically, and the imaging procedure repeated with the same settings. After imaging each animal, the tumors were excised and processed for histological analysis as described below. Prior to sectioning, the paraffin blocks were subjected to Raman imaging (wavelength 785 nm, objective ×20, power 134 mW, acquisition 100 ms, resolution 40 µm) to acquire the high-resolution images for histological comparison.

Misra V.K, & Draper D.E. (2001). A thermodynamic framework for Mg2+ binding to RNA. Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12456-12461.

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