Minimum essential medium non essential amino acids solutions

Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) and FBS were purchased from Corning (Tewksbury, MA, USA) and Biosera (Nuaille, France), respectively. Stable glutamine and Minimum Essential Medium non-essential amino acids solutions were obtained from Pan Biotech (Aidenbach, Germany). Insulin, resazurin based cell viability kit (TOX-8), Triton X-100, phosphatase inhibitor cocktail 2, and western blot reagents and buffer components were purchased from Sigma (St. Louis, MO, USA). CytoTox-ONE LDH Assay kit was supplied by Promega (Madison, WI, USA). STZ was obtained from Cayman Chemical Company (Ann Arbor, MI, USA) and was dissolved freshly in citrate buffer (0.1 M, pH 4.5) immediately before adding to the medium. DuoSet IC, Phospho-GSK-3α/β (S21/S9) and anti-GAPDH specific mouse monoclonal antibodies were provided by R&D Systems GmbH (Wiesbaden, Germany).

Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) and fetal bovine serum (FBS) were obtained from Corning (Tewksbury, MA, USA) and Biosera (Nuaille, France), respectively. Stable glutamine and Minimum Essential Medium non-essential amino acids solutions were purchased from Pan Biotech (Aidenbach, Germany). Insulin, Ex-4, resazurin based cell viability kit (TOX-8), Triton X-100, Phosphatase inhibitor cocktail 2 were obtained from Sigma (St. Louis, MO, USA). STZ was purchased from Cayman Chemical Company (Ann Arbor, MI, USA) and was dissolved in citrate buffer (0.1 M, pH 4.5) immediately before adding to the medium. DuoSet IC, Phospho-GSK-3α/β (S21/S9) ELISA kit was provided by R&D Systems GmbH (Wiesbaden, Germany).