D700 reflex camera

Pristine GO and nGO-PEG nanosheets were streaked on three different types of agar plates, including Columbia agar with 5% Sheep Blood (Becton Dickinson) for Gram (+) and Gram (–) microorganisms, MacConkey agar (Becton Dickinson)—a selective and differentiating agar for gram-negative bacterial species, and Sabouraud Dextrose Agar (Becton Dickinson)—a nonselective medium for pathogenic and non-pathogenic fungi, particularly dermatophytes. Staphylococcus aureus, Escherichia coli, and Candida albicans were used as positive controls of bacterial and fungal growth. Agar plates were incubated for 72 h at 37 °C. Microbial and fungal cell growth was evaluated and results are photographed with a Nikon D700 reflex camera (Nikon, Tokyo, Japan).

GO, rGO, and rGO-PEG nanosheets were streaked on Luria broth (LB) (Sigma-Aldrich, Germany) agar plates in a dilution of 1:200 in ddH₂O. Escherichia coli (strain DH5α) was used as a positive control of bacterial growth. Agar plates were incubated for 72 h at 37 °C. Macrophotographs were taken with a Nikon D700 reflex camera (Nikon, Tokio, Japan) and were processed using Adobe Photoshop CS5.