Fv3000
The FV3000 is a high-performance confocal microscope system designed for advanced imaging applications. It features a modular architecture, allowing for customization to meet specific research needs. The FV3000 delivers exceptional optical performance, enabling high-resolution, multi-dimensional imaging of a variety of sample types.
Lab products found in correlation
5 protocols using fv3000
Confocal Microscopy Imaging Protocol
Two-color Somite Fate Mapping in Skate Embryos
Injected skate embryos were euthanized using an overdose of tricaine (1 g/L in seawater) and fixed in 4% paraformaldehyde overnight at 4°C. Embryos were then rinsed 3 × 5 min in phosphate-buffered saline (PBS), embedded in 15% gelatin in PBS and post-fixed in 4% paraformaldehyde in PBS for 4 nights at 4°C before vibratome sectioning. A Leica VT1000S vibratome was used to cut 100 µm sections of tissue in sagittal plane, which were then DAPI-stained (1 µg/mL), coverslipped with Fluoromount-G (Southern Biotech) and imaged on an Olympus FV3000 (trunk and transitional vertebrae) or Leica Sp5 (tail vertebrae) confocal microscope.
Visualizing Plant Cell Walls and Nuclei
Three confocal microscopy systems were used for image collection in this study, namely the Olympus FV1000 and FV3000 systems and a Leica SP8 system. Imaging settings were as follows: PI, excitation: 552 nm, emission window: 570–670 nm; calcofluor white, excitation: 408 nm; emission window: 425–460 nm; GFP, excitation: 488 nm, emission window: 497–527 nm.
Whole-animal Fluorescent Imaging
Immunostaining of Liver Organoids
Immunostaining of mouse liver tissue slides for markers, including cytokeratin 19 (CK19/Krt19), albumin (Alb), α-SMA, and IL-17A, was performed using a Novo-Light Multiplex Fluorescence Immunohistochemical Kit (WiSee Bio, China). The complete list of immunostaining antibodies used can be found in Table S1.
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